Optimized criteria for locomotion-based healthspan evaluation in C. elegans using the WorMotel system.
Aging
/ physiology
Animals
Animals, Genetically Modified
Caenorhabditis elegans
/ genetics
Caenorhabditis elegans Proteins
/ antagonists & inhibitors
Forkhead Transcription Factors
/ antagonists & inhibitors
Gene Knockout Techniques
Healthy Aging
/ physiology
Humans
Locomotion
/ physiology
Longevity
/ physiology
Models, Animal
Models, Biological
RNA Interference
Receptor, Insulin
/ antagonists & inhibitors
Time Factors
Time-Lapse Imaging
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
13
08
2019
accepted:
11
02
2020
entrez:
4
3
2020
pubmed:
4
3
2020
medline:
17
6
2020
Statut:
epublish
Résumé
Getting a grip on how we may age healthily is a central interest of biogerontological research. To this end, a number of academic teams developed platforms for life- and healthspan assessment in Caenorhabditis elegans. These are very appealing for medium- to high throughput screens, but a broader implementation is lacking due to many systems relying on custom scripts for data analysis that others struggle to adopt. Hence, user-friendly recommendations would help to translate raw data into interpretable results. The aim of this communication is to streamline the analysis of data obtained by the WorMotel, an economically and practically appealing screening platform, in order to facilitate the use of this system by interested researchers. We here detail recommendations for the stepwise conversion of raw image data into activity values and explain criteria for assessment of health in C. elegans based on locomotion. Our analysis protocol can easily be adopted by researchers, and all needed scripts and a tutorial are available in S1 and S2 Files.
Identifiants
pubmed: 32126105
doi: 10.1371/journal.pone.0229583
pii: PONE-D-19-22873
pmc: PMC7053758
doi:
Substances chimiques
Caenorhabditis elegans Proteins
0
Forkhead Transcription Factors
0
daf-16 protein, C elegans
0
DAF-2 protein, C elegans
EC 2.7.10.1
Receptor, Insulin
EC 2.7.10.1
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0229583Subventions
Organisme : NIH HHS
ID : P40 OD010440
Pays : United States
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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