The gut anti-complement activity of Aedes aegypti: Investigating new ways to control the major human arboviruses vector in the Americas.
Aedes
/ microbiology
Americas
Animals
Chikungunya Fever
/ prevention & control
Chikungunya virus
/ physiology
Complement Inactivator Proteins
/ metabolism
Dengue
/ prevention & control
Dengue Virus
/ physiology
Female
Gastrointestinal Microbiome
/ physiology
Male
Mosquito Vectors
/ microbiology
Zika Virus
/ physiology
Zika Virus Infection
/ prevention & control
Aedes aegypti
C4 cleavage
Classical pathway
Factor H
Gut
Human complement
Journal
Insect biochemistry and molecular biology
ISSN: 1879-0240
Titre abrégé: Insect Biochem Mol Biol
Pays: England
ID NLM: 9207282
Informations de publication
Date de publication:
05 2020
05 2020
Historique:
received:
23
09
2019
revised:
14
02
2020
accepted:
20
02
2020
pubmed:
4
3
2020
medline:
7
10
2020
entrez:
4
3
2020
Statut:
ppublish
Résumé
Aedes aegypti is the main urban vector of dengue virus, chikungunya virus and Zika virus due to its great dispersal capacity and virus susceptibility. A. aegypti feed on plant-derived sugars but females need a blood meal for egg maturation. Haematophagous arthropods need to overcome host haemostasis and local immune reactions in order to take a blood meal. In this context, molecules present in the saliva and/or intestinal contents of these arthropods must contain inhibitors of the complement system (CS). CS salivary and/or intestinal inhibitors are crucial to protect gut cells of haematophagous arthropods against complement attack. The present work aimed to investigate the anti-complement activity of A. aegypti intestinal contents on the alternative, classical and lectin pathways of the human complement system. Here we show that A. aegypti gut contents inhibited the human classical and the lectin pathways but not the alternative pathway. The A. aegypti gut content has a serine protease able to specifically cleave and inactivate human C4, which is a novel mechanism for human complement inactivation in haematophagous arthropods. The gut of female A. aegypti was capable of capturing human serum factor H (a negative complement modulator), unlike males. C3 molecules in recently blood-fed female A. aegypti remain in their original state, being inactivated to iC3b soon after a blood feed. A transmission-blocking vaccine using these complement inhibitory proteins as antigens has the potential to interfere with the insect's survival, reproductive fitness and block their infection by the arboviruses they transmit to humans.
Identifiants
pubmed: 32126277
pii: S0965-1748(20)30027-8
doi: 10.1016/j.ibmb.2020.103338
pii:
doi:
Substances chimiques
Complement Inactivator Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
103338Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing Interest The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.