C - reactive protein of cerebrospinal fluid, as a sensitive approach for diagnosis of neonatal meningitis.


Journal

African health sciences
ISSN: 1729-0503
Titre abrégé: Afr Health Sci
Pays: Uganda
ID NLM: 101149451

Informations de publication

Date de publication:
Sep 2019
Historique:
entrez: 5 3 2020
pubmed: 5 3 2020
medline: 1 9 2020
Statut: ppublish

Résumé

Meningitis, is a potentially life-threatening condition that can rapidly progress to permanent brain damage, neurologic problems, and even death. Bacteria and viruses cause the great majority of meningitis disease in infants and children. CRP is used mainly as a marker of inflammation. This study was conducted to assess the diagnostic value of CSF-CRP levels for differentiating between septic (bacterial) and aseptic infantile meningitis. 49 hospitalized infants aged less than two months with suspected meningitis were enrolled in a cross-sectional analytic study. All of patients underwent lumbar puncture to obtain CSF. smears, cultures, cytological and biochemical analysis and latex agglutination testing were carried out on all CSF samples. Latex agglutination test was carried out on all CSF samples using a commercially available kit. CSF-CRP level of all infants was measured using the immunoturbidometric technique. Of 49 infants in this study, 20 and 29 cases were diagnosed as septic and aseptic meningitis, respectively. The CRP levels were obtained as 0.95±0.68 mg/L in septic and 0.16±0.36 mg/L in aseptic meningitis groups and this difference was statistically significant (p<0.001) between the two groups (0.79±0.32 mg/L). Based on the ROC curve, cut off levels for CRP was obtained 0.17 mg/L. At this level, there was 95% sensitivity and 86% specificity to differentiate septic and aseptic meningitis. CSF-CRP has suitable diagnostic value in distinguishing between infantile bacterial from aseptic meningitis especially in cases of negative bacterial culture of the blood and spinal fluid.

Sections du résumé

BACKGROUND BACKGROUND
Meningitis, is a potentially life-threatening condition that can rapidly progress to permanent brain damage, neurologic problems, and even death. Bacteria and viruses cause the great majority of meningitis disease in infants and children. CRP is used mainly as a marker of inflammation.
OBJECTIVE OBJECTIVE
This study was conducted to assess the diagnostic value of CSF-CRP levels for differentiating between septic (bacterial) and aseptic infantile meningitis.
METHODS METHODS
49 hospitalized infants aged less than two months with suspected meningitis were enrolled in a cross-sectional analytic study. All of patients underwent lumbar puncture to obtain CSF. smears, cultures, cytological and biochemical analysis and latex agglutination testing were carried out on all CSF samples. Latex agglutination test was carried out on all CSF samples using a commercially available kit. CSF-CRP level of all infants was measured using the immunoturbidometric technique.
RESULTS RESULTS
Of 49 infants in this study, 20 and 29 cases were diagnosed as septic and aseptic meningitis, respectively. The CRP levels were obtained as 0.95±0.68 mg/L in septic and 0.16±0.36 mg/L in aseptic meningitis groups and this difference was statistically significant (p<0.001) between the two groups (0.79±0.32 mg/L). Based on the ROC curve, cut off levels for CRP was obtained 0.17 mg/L. At this level, there was 95% sensitivity and 86% specificity to differentiate septic and aseptic meningitis.
CONCLUSION CONCLUSIONS
CSF-CRP has suitable diagnostic value in distinguishing between infantile bacterial from aseptic meningitis especially in cases of negative bacterial culture of the blood and spinal fluid.

Identifiants

pubmed: 32127807
doi: 10.4314/ahs.v19i3.10
pii: jAFHS.v19.i3.pg2372
pmc: PMC7040284
doi:

Substances chimiques

Biomarkers 0
C-Reactive Protein 9007-41-4

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

2372-2377

Informations de copyright

© 2019 Javadinia et al.

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Auteurs

Shima Javadinia (S)

Department of Medicine, School of Medicine, Zabol University of Medical Sciences, Zabol, Iran.

Mohsen Tabasi (M)

Legal Medicine Research Center, Legal Medicine Organization, Tehran, Iran.

Mehri Naghdalipour (M)

Research Center of Pediatric infectious diseases, Rasoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran.

Najmosadat Atefi (N)

Research Center of Pediatric infectious diseases, Rasoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran.

Ramin Asgarian (R)

Research Center of Pediatric infectious diseases, Rasoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran.

Jamil Kheirvari Khezerloo (JK)

Young Researchers and Elite Club, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.

Azardokht Tabatabaei (A)

Research Center of Pediatric infectious diseases, Rasoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran.

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Classifications MeSH