Microcircuit Rules Governing Impact of Single Interneurons on Purkinje Cell Output In Vivo.
Purkinje cell
cerebellum
climbing fiber
glutamate spillover
in vivo
inhibition
interneuron
patch clamp
synaptic integration
two-photon imaging
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
03 03 2020
03 03 2020
Historique:
received:
07
10
2017
revised:
07
01
2020
accepted:
03
02
2020
entrez:
5
3
2020
pubmed:
5
3
2020
medline:
25
3
2021
Statut:
ppublish
Résumé
The functional impact of single interneurons on neuronal output in vivo and how interneurons are recruited by physiological activity patterns remain poorly understood. In the cerebellar cortex, molecular layer interneurons and their targets, Purkinje cells, receive excitatory inputs from granule cells and climbing fibers. Using dual patch-clamp recordings from interneurons and Purkinje cells in vivo, we probe the spatiotemporal interactions between these circuit elements. We show that single interneuron spikes can potently inhibit Purkinje cell output, depending on interneuron location. Climbing fiber input activates many interneurons via glutamate spillover but results in inhibition of those interneurons that inhibit the same Purkinje cell receiving the climbing fiber input, forming a disinhibitory motif. These interneuron circuits are engaged during sensory processing, creating diverse pathway-specific response functions. These findings demonstrate how the powerful effect of single interneurons on Purkinje cell output can be sculpted by various interneuron circuit motifs to diversify cerebellar computations.
Identifiants
pubmed: 32130904
pii: S2211-1247(20)30163-7
doi: 10.1016/j.celrep.2020.02.009
pmc: PMC7059114
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
3020-3035.e3Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 201225/Z/16/Z
Pays : United Kingdom
Informations de copyright
Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests.
Références
Trends Neurosci. 2010 Jun;33(6):259-66
pubmed: 20371122
Nat Neurosci. 2015 Dec;18(12):1798-803
pubmed: 26551541
J Neurosci. 2011 Jul 27;31(30):10847-58
pubmed: 21795537
Neuron. 2000 Nov;28(2):547-58
pubmed: 11144363
Curr Neuropharmacol. 2010 Mar;8(1):2-9
pubmed: 20808541
J Neurosci Methods. 2000 Nov 30;103(2):199-208
pubmed: 11084213
Neuron. 2014 Feb 19;81(4):913-29
pubmed: 24559679
J Physiol. 2016 May 15;594(10):2729-49
pubmed: 26918702
Science. 2015 Nov 27;350(6264):aac9462
pubmed: 26612957
J Neurosci. 2012 Dec 12;32(50):17988-97
pubmed: 23238715
J Physiol. 2010 Oct 1;588(Pt 19):3757-83
pubmed: 20724365
J Neurosci. 2014 Nov 5;34(45):14984-94
pubmed: 25378164
Neuron. 2018 Aug 8;99(3):564-575.e2
pubmed: 30017394
Neuron. 2019 Aug 7;103(3):506-519.e4
pubmed: 31201123
Nat Neurosci. 2009 Aug;12(8):1042-9
pubmed: 19578381
Nat Neurosci. 2014 Feb;17(2):289-95
pubmed: 24413696
Proc Natl Acad Sci U S A. 2007 May 1;104(18):7658-63
pubmed: 17452649
J Neurosci. 2009 Aug 19;29(33):10234-53
pubmed: 19692598
Neuron. 1997 Feb;18(2):281-93
pubmed: 9052798
J Neurosci. 2002 Aug 15;22(16):7055-64
pubmed: 12177202
J Physiol. 2005 Mar 1;563(Pt 2):369-78
pubmed: 15613376
Neuron. 1993 Oct;11(4):759-69
pubmed: 8398158
J Neurophysiol. 2000 Apr;83(4):1932-40
pubmed: 10758104
J Physiol. 1979 Apr;289:425-48
pubmed: 458677
J Comp Neurol. 2001 Jan 1;429(1):59-70
pubmed: 11086289
J Neurosci. 1997 Jan 1;17(1):91-106
pubmed: 8987739
Sci Rep. 2019 Feb 11;9(1):1742
pubmed: 30742002
Biomed Eng Online. 2003 May 17;2:13
pubmed: 12801419
Neuron. 2018 Sep 5;99(5):999-1015.e6
pubmed: 30122378
PLoS One. 2012;7(5):e37031
pubmed: 22623975
J Comp Neurol. 1999 Jun 14;408(4):580-96
pubmed: 10340507
Neuron. 2011 Oct 20;72(2):231-43
pubmed: 22017986
J Neurosci. 2009 Nov 11;29(45):14352-62
pubmed: 19906982
Nat Neurosci. 2007 Jun;10(6):735-42
pubmed: 17515900
Nat Methods. 2008 Jan;5(1):61-7
pubmed: 18157136
Neuron. 2013 May 22;78(4):700-13
pubmed: 23643935
J Neurosci. 1999 Jul 1;19(13):5265-74
pubmed: 10377338
J Neurosci. 2010 Jun 16;30(24):8171-9
pubmed: 20554867
Nat Rev Neurosci. 2011 Jun;12(6):327-44
pubmed: 21544091
Neuron. 2016 May 18;90(4):810-23
pubmed: 27161527
Proc Natl Acad Sci U S A. 2010 Jul 20;107(29):13153-8
pubmed: 20615960
Neuron. 2003 Sep 11;39(6):911-8
pubmed: 12971892
Nature. 2011 Dec 07;480(7377):331-5
pubmed: 22158104
J Neurosci. 1997 Dec 1;17(23):9104-12
pubmed: 9364057
Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11569-73
pubmed: 1763072
Nature. 1995 Nov 2;378(6552):75-8
pubmed: 7477292
J Neurophysiol. 1999 Apr;81(4):1531-47
pubmed: 10200189
Nat Commun. 2015 Mar 09;6:6464
pubmed: 25751648
Neuron. 2013 Jun 19;78(6):1050-62
pubmed: 23707614
Neuron. 2002 May 30;34(5):797-806
pubmed: 12062025
J Neurosci. 1995 Apr;15(4):2777-87
pubmed: 7722628
J Physiol. 2004 Feb 1;554(Pt 3):707-20
pubmed: 14617674
Elife. 2018 Apr 16;7:
pubmed: 29659351
Nat Neurosci. 2002 Dec;5(12):1309-18
pubmed: 12411959
J Physiol. 1998 May 15;509 ( Pt 1):221-32
pubmed: 9547395
Front Neural Circuits. 2013 Apr 19;7:73
pubmed: 23626524
J Neurosci. 1997 Jun 15;17(12):4517-26
pubmed: 9169512
Trends Cogn Sci. 1998 Sep 1;2(9):322-30
pubmed: 21227228
Elife. 2015 Jun 17;4:
pubmed: 26083712
J Neurosci. 2007 May 23;27(21):5559-70
pubmed: 17522301
Nature. 2018 Nov;563(7729):113-116
pubmed: 30333626
Cell Rep. 2015 Dec 1;13(9):1977-88
pubmed: 26655909
J Neurosci. 2003 Oct 22;23(29):9620-31
pubmed: 14573542
J Neurosci. 2011 Jul 20;31(29):10463-73
pubmed: 21775592
Neuron. 2018 Nov 7;100(3):564-578.e3
pubmed: 30293822
J Comp Neurol. 1998 Apr 13;393(3):353-73
pubmed: 9548555
Neuron. 1996 Apr;16(4):815-23
pubmed: 8607999
J Neurosci. 1999 Apr 15;19(8):2876-86
pubmed: 10191305
Nat Methods. 2012 Jul;9(7):671-5
pubmed: 22930834
Front Syst Neurosci. 2013 May 17;7:16
pubmed: 23730272
Nature. 2014 May 22;509(7501):453-8
pubmed: 24814341
J Neurosci. 1999 May 1;19(9):3298-306
pubmed: 10212289
Neuron. 2014 Feb 5;81(3):653-63
pubmed: 24507196
J Neurosci. 2006 Aug 9;26(32):8267-77
pubmed: 16899721
Exp Brain Res. 1966;1(1):1-16
pubmed: 5910941
J Neurophysiol. 2004 Jul;92(1):600-8
pubmed: 15056678
Cell Rep. 2015 Dec 1;13(9):1741-6
pubmed: 26655894
Neuron. 1997 Sep;19(3):665-78
pubmed: 9331356
Neuron. 2016 Jul 20;91(2):260-92
pubmed: 27477017
Elife. 2018 Aug 17;7:
pubmed: 30117806