Vibration acceleration promotes endochondral formation during fracture healing through cellular chondrogenic differentiation.
Acceleration
Animals
Biomechanical Phenomena
/ physiology
Bony Callus
/ physiology
Cell Differentiation
Cells, Cultured
Chondrocytes
/ physiology
Chondrogenesis
/ physiology
Female
Fracture Healing
/ physiology
Fractures, Bone
/ physiopathology
Osteogenesis
/ physiology
Rats
Rats, Wistar
Vibration
/ therapeutic use
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
31
05
2019
accepted:
30
01
2020
entrez:
6
3
2020
pubmed:
7
3
2020
medline:
18
6
2020
Statut:
epublish
Résumé
Vibration acceleration through whole body vibration has been reported to promote fracture healing. However, the mechanism responsible for this effect remains unclear. Purpose of this study was to determine whether vibration acceleration directly affects cells around the fracture site and promotes endochondral ossification. Four-week-old female Wistar Hannover rats were divided into two groups (vibration [V group] and control [C group]). The eighth ribs on both sides were cut vertically using scissors. From postoperative day 3 to 11, vibration acceleration using Power Plate® (30 Hz, low amplitude [30-Low], 10 min/day) was applied in the V group. Mature calluses appeared earlier in the V group than in the C group by histological analysis. The GAG content in the fracture callus on day 6 was significantly higher in the V group than in the C group. The mRNA expressions of SOX-9, aggrecan, and Col-II in the fracture callus on day 6 and Col-X on day 9 were significantly higher in the V group than in the C group. For in vitro analysis, four different conditions of vibration acceleration (30 or 50 Hz with low or high amplitude [30-Low, 30-High, 50-Low, and 50-High], 10 min/day) were applied to a prechondrogenic cell (ATDC5) and an undifferentiated cell (C3H10T1/2). There was no significant difference in cell proliferation between the control and any of the four vibration conditions for both cell lines. For both cell lines, alcian blue staining was greater under 30-Low and 50-Low conditions than under control as well as 30-High and 50-High conditions on days 7 and 14. Vibration acceleration under 30-L condition upregulated chondrogenic gene expressions of SOX-9, aggrecan, Col-II, and Col-X. Low-amplitude vibration acceleration can promote endochondral ossification in the fracture healing in vivo and chondrogenic differentiation in vitro.
Identifiants
pubmed: 32134943
doi: 10.1371/journal.pone.0229127
pii: PONE-D-19-12484
pmc: PMC7058294
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0229127Déclaration de conflit d'intérêts
This manuscript was supported by PROTEA JAPAN Company, Limited [http://www.protea.co.jp], and grant number J167701901. This does not alter our adherence to PLOS ONE policies on sharing data and materials.
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