Starvation-induced autophagy via calcium-dependent TFEB dephosphorylation is suppressed by Shigyakusan.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
23
10
2019
accepted:
22
02
2020
entrez:
6
3
2020
pubmed:
7
3
2020
medline:
23
6
2020
Statut:
epublish
Résumé
Kampo, a system of traditional Japanese therapy utilizing mixtures of herbal medicine, is widely accepted in the Japanese medical system. Kampo originated from traditional Chinese medicine, and was gradually adopted into a Japanese style. Although its effects on a variety of diseases are appreciated, the underlying mechanisms remain mostly unclear. Using a quantitative tf-LC3 system, we conducted a high-throughput screen of 128 kinds of Kampo to evaluate the effects on autophagy. The results revealed a suppressive effect of Shigyakusan/TJ-35 on autophagic activity. TJ-35 specifically suppressed dephosphorylation of ULK1 and TFEB, among several TORC1 substrates, in response to nutrient deprivation. TFEB was dephosphorylated by calcineurin in a Ca2+ dependent manner. Cytosolic Ca2+ concentration was increased in response to nutrient starvation, and TJ-35 suppressed this increase. Thus, TJ-35 prevents the starvation-induced Ca2+ increase, thereby suppressing induction of autophagy.
Identifiants
pubmed: 32134989
doi: 10.1371/journal.pone.0230156
pii: PONE-D-19-29517
pmc: PMC7058311
doi:
Substances chimiques
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
0
Drugs, Chinese Herbal
0
TFEB protein, human
0
shigyaku-san
0
Mechanistic Target of Rapamycin Complex 1
EC 2.7.11.1
Calcineurin
EC 3.1.3.16
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0230156Déclaration de conflit d'intérêts
Takeshi NODA has received research grant from Tsumura CO. LTD. Tsumura CO LTD has no role in the study design; collection, analysis, and interpretation of data; writing of the paper; and decision to submit for publication. This research is not related to any of employment, consultancy, patents, products in development of Tsumura CO. LTD. This does not alter the authors' adherence to all the PLoS ONE policies on sharing data and materials. The other authors have nothing to disclose.
Références
Cell Calcium. 2012 Aug;52(2):170-81
pubmed: 22721780
Autophagy. 2016 Aug 2;12(8):1355-71
pubmed: 25905985
J Biol Chem. 2003 Sep 19;278(38):36005-12
pubmed: 12840022
Mol Biol Cell. 2011 Jul 1;22(13):2290-300
pubmed: 21525242
Biomed Res Int. 2014;2014:832704
pubmed: 25013806
Cell Calcium. 2011 Sep;50(3):242-50
pubmed: 21571367
Mol Cell Biol. 2004 Jan;24(1):200-16
pubmed: 14673156
Science. 2009 Jul 24;325(5939):473-7
pubmed: 19556463
Medicines (Basel). 2019 Jun 03;6(2):
pubmed: 31163644
J Cell Biol. 2008 Jun 30;181(7):1065-72
pubmed: 18591426
Proc Natl Acad Sci U S A. 2010 Apr 13;107(15):6970-5
pubmed: 20351288
Phytother Res. 2006 Apr;20(4):256-62
pubmed: 16557606
Br J Pharmacol. 2002 Apr;135(8):1959-66
pubmed: 11959799
Int J Cell Biol. 2012;2012:736905
pubmed: 22518139
Methods Enzymol. 2009;452:1-12
pubmed: 19200872
Curr Opin Cell Biol. 2010 Apr;22(2):132-9
pubmed: 20056399
J Cell Biol. 2013 Feb 18;200(4):475-91
pubmed: 23401004
Nat Cell Biol. 2018 May;20(5):521-527
pubmed: 29686264
J Cell Sci. 2015 Jan 15;128(2):207-17
pubmed: 25568150
Mol Cell. 2019 Jan 17;73(2):339-353.e6
pubmed: 30581147
Nat Med. 2019 Apr;25(4):620-627
pubmed: 30833748
EMBO J. 2011 Jul 29;30(16):3242-58
pubmed: 21804531
Biomolecules. 2017 Jul 07;7(3):
pubmed: 28686223
Cell. 1991 Sep 6;66(5):823-6
pubmed: 1716181
J Cell Sci. 2018 Jan 29;131(3):
pubmed: 29222112
Curr Biol. 2002 Apr 16;12(8):632-9
pubmed: 11967149
Nat Biotechnol. 2001 Feb;19(2):137-41
pubmed: 11175727
Nat Cell Biol. 2015 Mar;17(3):288-99
pubmed: 25720963
Sci Signal. 2009 Aug 18;2(84):pe51
pubmed: 19690328
PLoS One. 2014 May 05;9(5):e96690
pubmed: 24798445
Autophagy. 2007 Sep-Oct;3(5):452-60
pubmed: 17534139
Autophagy. 2011 Dec;7(12):1472-89
pubmed: 22082873
Cancer Sci. 2018 Oct;109(10):3055-3067
pubmed: 30033530
Pharmacology. 2017;99(3-4):99-105
pubmed: 27788517
Mol Cell. 2007 Jan 26;25(2):193-205
pubmed: 17244528
J Cell Biol. 2008 May 5;181(3):497-510
pubmed: 18443221
Curr Opin Cell Biol. 2019 Dec;61:117-125
pubmed: 31480011
Nat Rev Drug Discov. 2012 Sep;11(9):709-30
pubmed: 22935804
J Int Med Res. 2006 May-Jun;34(3):231-9
pubmed: 16866016
J Ethnopharmacol. 2016 Dec 24;194:861-876
pubmed: 27793785
J Clin Invest. 2015 Jan;125(1):1-4
pubmed: 25654544
Science. 1999 Sep 24;285(5436):2129-33
pubmed: 10497131
Science. 2011 Jun 17;332(6036):1429-33
pubmed: 21617040
Cell Struct Funct. 1998 Feb;23(1):33-42
pubmed: 9639028
J Cell Biol. 2001 Feb 19;152(4):657-68
pubmed: 11266458
Autophagy. 2005 Jul;1(2):84-91
pubmed: 16874052
Eye (Lond). 2017 Jun;31(6):828-845
pubmed: 28282061
J Biol Chem. 2009 Mar 20;284(12):8023-32
pubmed: 19150980
FEBS Lett. 1982 Jan 11;137(1):80-4
pubmed: 6279434
Mol Biol Cell. 2009 Apr;20(7):1981-91
pubmed: 19211835