Feed Additives Differentially Impact the Epimural Microbiota and Host Epithelial Gene Expression of the Bovine Rumen Fed Diets Rich in Concentrates.
autolyzed yeast
feed additives
gene expression
phytogenics
rumen epithelial microbiome
Journal
Frontiers in microbiology
ISSN: 1664-302X
Titre abrégé: Front Microbiol
Pays: Switzerland
ID NLM: 101548977
Informations de publication
Date de publication:
2020
2020
Historique:
received:
01
10
2019
accepted:
20
01
2020
entrez:
7
3
2020
pubmed:
7
3
2020
medline:
7
3
2020
Statut:
epublish
Résumé
The success of nutritional strategies for the prevention of subacute ruminal acidosis (SARA) and the related microbial dysbiosis still remains unpredictable due to the complexity of the rumen ecosystem. The rumen epimural community, due to proximity, has the greatest opportunity to influence host gene expression. The aim of this study was to determine the effect of two separate feed additives on the rumen epimural community and host epithelial gene expression. Eight rumen cannulated Holstein cows were randomly assigned to one of three feeding groups: autolyzed yeast (AY), phytogenics (PHY) and control (CON) using a 3 × 3 Latin square design. Cows were fed an intermittent SARA model that started with 100% forage diet (Baseline) followed by two 65% concentrate-diet induced SARA challenges (SARAI, SARAII), separated by 1 week of forage only feeding. Rumen papillae samples were collected via the cannula during the Baseline, SARAI and SARAII periods. Microbial DNA was extracted and sequenced targeting the 16S rRNA gene and host RNA was analyzed using RT-qPCR. Analysis of the taxonomic composition at the genera level showed a tendency to increase in the relative abundances of
Identifiants
pubmed: 32140139
doi: 10.3389/fmicb.2020.00119
pmc: PMC7043141
doi:
Types de publication
Journal Article
Langues
eng
Pagination
119Informations de copyright
Copyright © 2020 Petri, Neubauer, Humer, Kröger, Reisinger and Zebeli.
Références
Front Microbiol. 2016 May 18;7:701
pubmed: 27242724
J Dairy Sci. 2008 May;91(5):2046-66
pubmed: 18420634
J Dairy Sci. 2019 Jun;102(6):5361-5378
pubmed: 31005320
Appl Environ Microbiol. 2018 May 31;84(12):
pubmed: 29654184
Can Vet J. 2000 May;41(5):388-94
pubmed: 10816832
J Dairy Sci. 2012 Jan;95(1):318-27
pubmed: 22192211
J Bacteriol. 2000 Feb;182(4):1154-7
pubmed: 10648544
J Dairy Sci. 2017 Mar;100(3):1829-1844
pubmed: 28041738
N Engl J Med. 2000 Aug 3;343(5):338-44
pubmed: 10922424
J Dairy Sci. 2015 Jan;98(1):586-94
pubmed: 25468705
J Vet Med A Physiol Pathol Clin Med. 2003 Oct;50(8):406-14
pubmed: 14633219
Methods Enzymol. 2013;531:371-444
pubmed: 24060131
Cancer Res. 2004 Aug 1;64(15):5245-50
pubmed: 15289330
Cytokine. 2010 Jan;49(1):1-9
pubmed: 19775907
Anaerobe. 2019 Oct;59:38-48
pubmed: 31102775
J Dairy Sci. 1997 May;80(5):1005-28
pubmed: 9178142
Appl Microbiol Biotechnol. 2012 Jul;95(2):289-97
pubmed: 22615053
J Oral Microbiol. 2014 Oct 08;6:
pubmed: 25317252
J Anim Sci. 2013 Jul;91(7):3291-8
pubmed: 23572258
BMC Vet Res. 2016 Jun 27;12(1):128
pubmed: 27350130
Bioinformatics. 2010 Oct 1;26(19):2460-1
pubmed: 20709691
Res Vet Sci. 2012 Dec;93(3):1099-108
pubmed: 22370295
Nat Protoc. 2008;3(6):1101-8
pubmed: 18546601
J Dairy Sci. 2018 Oct;101(10):9559-9574
pubmed: 30031584
Nucleic Acids Res. 2013 Jan;41(Database issue):D590-6
pubmed: 23193283
PLoS One. 2013 Dec 31;8(12):e83424
pubmed: 24391765
J Dairy Sci. 2018 Mar;101(3):2335-2349
pubmed: 29331466
Sci Rep. 2019 Jan 15;9(1):120
pubmed: 30644398
PLoS One. 2017 Aug 1;12(8):e0182271
pubmed: 28763489
Nat Rev Immunol. 2006 Jan;6(1):33-43
pubmed: 16341139
J Dairy Sci. 2015 Oct;98(10):6631-50
pubmed: 26210279
J Dairy Sci. 1997 Sep;80(9):2035-44
pubmed: 9313145
Appl Environ Microbiol. 1980 May;39(5):1059-66
pubmed: 6772103
Biotechnol Lett. 2004 Mar;26(6):509-15
pubmed: 15127793
PLoS One. 2011 Apr 04;6(4):e18573
pubmed: 21483702
Front Microbiol. 2015 Mar 02;6:167
pubmed: 25784904
Am J Physiol Regul Integr Comp Physiol. 2011 Jun;300(6):R1515-23
pubmed: 21451145
J Anim Sci. 2013 Sep;91(9):4256-66
pubmed: 23825322
J Dairy Sci. 2011 Aug;94(8):4046-56
pubmed: 21787940
Genome Biol. 2011 Jun 24;12(6):R60
pubmed: 21702898
Int J Syst Bacteriol. 1995 Apr;45(2):297-300
pubmed: 7537062
Nat Methods. 2013 Jan;10(1):57-9
pubmed: 23202435
Nat Rev Immunol. 2004 Jul;4(7):499-511
pubmed: 15229469
J Dairy Sci. 1988 Nov;71(11):2967-75
pubmed: 3230186
J Dairy Sci. 2017 Dec;100(12):9702-9714
pubmed: 28964521
Nat Methods. 2010 May;7(5):335-6
pubmed: 20383131
Genome Res. 2009 Dec;19(12):2317-23
pubmed: 19819907