Seroprevalence of Sarcocystis falcatula in Two Islands of Malaysia using Recombinant Surface Antigen 4.
Sarcocystis falcatula
sarcocystosis
serodiagnosis
surface antigen
Journal
The Korean journal of parasitology
ISSN: 1738-0006
Titre abrégé: Korean J Parasitol
Pays: Korea (South)
ID NLM: 9435800
Informations de publication
Date de publication:
Feb 2020
Feb 2020
Historique:
received:
29
07
2019
accepted:
06
11
2019
entrez:
9
3
2020
pubmed:
9
3
2020
medline:
7
8
2020
Statut:
ppublish
Résumé
Sarcocystosis was diagnosed worldwide by serodiagnostic tests utilising the whole parasite, for which the protozoa were maintained in vitro are more costly. In this study, antigenicity of Sarcocystis falcatula recombinant protein (rSfSAG4) was investigated towards the local communities of Pangkor and Tioman Islands and its seroprevalence was surveyed in these islands. A total of 348 human sera were tested using rSfSAG4 by Western blot and ELISA. High prevalence of sarcocystosis was observed in Tioman Island (80.6%) than in Pangkor Island (50.0%) by Western blot. In ELISA, the seroprevalence observed in Tioman Island was 45.9%, whereas in Pangkor Island 63.0%. In other parasitic infections, the prevalence was 34.0% by Western blot and 46.0% by ELISA. In healthy control group, 7% by Western blot and 8% by ELISA showed positivity to rSfSAG4. It is suggested SfSAG4 is a candidate antigen to measure seroprevalence of sarcocystosis.
Identifiants
pubmed: 32145721
pii: kjp.2020.58.1.1
doi: 10.3347/kjp.2020.58.1.1
pmc: PMC7066447
doi:
Substances chimiques
Antigens, Protozoan
0
Antigens, Surface
0
Recombinant Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1-5Subventions
Organisme : Ministry of Higher Education, Malaysia
ID : PG033-2016A
Organisme : University of Malaya
ID : BKS001-2017
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