A critical review of recent trends, and a future perspective of optical spectroscopy as PAT in biopharmaceutical downstream processing.


Journal

Analytical and bioanalytical chemistry
ISSN: 1618-2650
Titre abrégé: Anal Bioanal Chem
Pays: Germany
ID NLM: 101134327

Informations de publication

Date de publication:
Apr 2020
Historique:
received: 25 10 2019
accepted: 10 01 2020
revised: 06 01 2020
pubmed: 9 3 2020
medline: 15 12 2020
entrez: 9 3 2020
Statut: ppublish

Résumé

As competition in the biopharmaceutical market gets keener due to the market entry of biosimilars, process analytical technologies (PATs) play an important role for process automation and cost reduction. This article will give a general overview and address the recent innovations and applications of spectroscopic methods as PAT tools in the downstream processing of biologics. As data analysis strategies are a crucial part of PAT, the review discusses frequently used data analysis techniques and addresses data fusion methodologies as the combination of several sensors is moving forward in the field. The last chapter will give an outlook on the application of spectroscopic methods in combination with chemometrics and model predictive control (MPC) for downstream processes. Graphical abstract.

Identifiants

pubmed: 32146498
doi: 10.1007/s00216-020-02407-z
pii: 10.1007/s00216-020-02407-z
pmc: PMC7072065
doi:

Substances chimiques

Biological Products 0

Types de publication

Journal Article Review

Langues

eng

Sous-ensembles de citation

IM

Pagination

2047-2064

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Auteurs

Laura Rolinger (L)

Institute of Engineering in Life Sciences, Section IV: Biomolecular Separation Engineering, Karlsruhe Institute of Technology, Fritz-Haber-Weg 2, 76131, Karlsruhe, Germany.

Matthias Rüdt (M)

Institute of Engineering in Life Sciences, Section IV: Biomolecular Separation Engineering, Karlsruhe Institute of Technology, Fritz-Haber-Weg 2, 76131, Karlsruhe, Germany.

Jürgen Hubbuch (J)

Institute of Engineering in Life Sciences, Section IV: Biomolecular Separation Engineering, Karlsruhe Institute of Technology, Fritz-Haber-Weg 2, 76131, Karlsruhe, Germany. juergen.hubbuch@kit.edu.

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