Quaternized styryl-azinium fluorophores as cellular RNA-binders.
Journal
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology
ISSN: 1474-9092
Titre abrégé: Photochem Photobiol Sci
Pays: England
ID NLM: 101124451
Informations de publication
Date de publication:
01 Mar 2020
01 Mar 2020
Historique:
pubmed:
10
3
2020
medline:
15
12
2020
entrez:
10
3
2020
Statut:
ppublish
Résumé
The capability of three quaternized styryl-azinium iodides to bind cellular RNA has been tested by means of Fluorescence Confocal Microscopy imaging of stained MCF-7 cells treated with RNase. Their association constants have been estimated through spectrophotometric and fluorimetric titrations with tRNA and compared to their affinity toward DNA. Transient absorption spectroscopy with femtosecond resolution confirmed the binding of the investigated compounds with tRNA and shed new light on the excited state dynamics of their complexes, by revealing a significant lengthening of the lifetime of S
Identifiants
pubmed: 32147676
doi: 10.1039/c9pp00465c
pii: 10.1039/c9pp00465c
doi:
Substances chimiques
Fluorescent Dyes
0
Pyrazines
0
Styrenes
0
RNA
63231-63-0
Ribonucleases
EC 3.1.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
362-370Références
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