Cancer stem cell enrichment is associated with enhancement of nicotinamide N-methyltransferase expression.
cancer stem cells
gene expression analysis
magnetic-activated cell sorting
molecular characterization
nicotinamide N-methyltransferase
sphere-forming cells
Journal
IUBMB life
ISSN: 1521-6551
Titre abrégé: IUBMB Life
Pays: England
ID NLM: 100888706
Informations de publication
Date de publication:
07 2020
07 2020
Historique:
received:
16
01
2020
revised:
17
02
2020
accepted:
19
02
2020
pubmed:
10
3
2020
medline:
16
11
2021
entrez:
10
3
2020
Statut:
ppublish
Résumé
The cancer stem cell theory states that a subset of tumor cells, termed cancer stem cells (CSCs), has the ability to self-renew and differentiate within the tumors. According to this theory, CSCs would be mainly responsible for tumor initiation, progression, resistance to therapy, recurrence, and metastasis. In this study, a culture system was setup to enrich CSCs from bladder cancer (T24), lung cancer (A549), colorectal cancer (CaCo-2), and osteosarcoma (MG63) cell lines, through sphere formation. Magnetic-activated cell sorting was also used to further increase CSC enrichment. Subsequently, molecular characterization of CSC-enriched cell populations and parental cells was carried out, by exploring the expression levels of stem markers and the enzyme nicotinamide N-methyltransferase (NNMT). Results obtained showed a significant upregulation of stem cell markers in CSC-enriched populations, obtained upon sphere formation, compared with parental counterparts. Moreover, NNMT expression levels were markedly increased in samples enriched with CSCs with respect to control cells. Considering the fundamental role played by CSCs in carcinogenesis, reported data strengthen the hypothesis that sustains a pivotal role of NNMT in cancer growth and metastasis. In addition, these findings could represent an important achievement for the development of new and effective anticancer therapies, based on CSC-associated targets.
Substances chimiques
NNMT protein, human
EC 2.1.1.1
Nicotinamide N-Methyltransferase
EC 2.1.1.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1415-1425Informations de copyright
© 2020 International Union of Biochemistry and Molecular Biology.
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