Structural model for differential cap maturation at growing microtubule ends.
GTP-cap
dynamic instability
microtubule
molecular biophysics
none
structural biology
taxol
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
10 03 2020
10 03 2020
Historique:
received:
12
07
2019
accepted:
25
01
2020
entrez:
11
3
2020
pubmed:
11
3
2020
medline:
31
3
2021
Statut:
epublish
Résumé
Microtubules (MTs) are hollow cylinders made of tubulin, a GTPase responsible for essential functions during cell growth and division, and thus, key target for anti-tumor drugs. In MTs, GTP hydrolysis triggers structural changes in the lattice, which are responsible for interaction with regulatory factors. The stabilizing GTP-cap is a hallmark of MTs and the mechanism of the chemical-structural link between the GTP hydrolysis site and the MT lattice is a matter of debate. We have analyzed the structure of tubulin and MTs assembled in the presence of fluoride salts that mimic the GTP-bound and GDP•P
Identifiants
pubmed: 32151315
doi: 10.7554/eLife.50155
pii: 50155
pmc: PMC7064335
doi:
pii:
Substances chimiques
Tubulin
0
Guanosine Diphosphate
146-91-8
Guanosine Triphosphate
86-01-1
Banques de données
PDB
['6gze', '6s9e', '4i55', '3ryh', '5xp3', '3jat', '3jal', '3jar', '3jak', '6evx', '2ngr', '1grn', '2b92', '2g77', '4jvs', '4iru']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Ministerio de Economía y Competitividad
ID : BFU2013-47014P
Organisme : Ministerio de Economía y Competitividad
ID : RYC-2011-07900
Organisme : Ministerio de Economía y Competitividad
ID : BFU2016-75319-R
Organisme : H2020 European Research Council
ID : H2020-MSCA-ITN-EJD-860070
Organisme : Swiss National Science Foundation
ID : 31003A_166608
Pays : Switzerland
Organisme : Japan Society for the Promotion of Science
ID : KAKENHI 16K07328/17H03668
Organisme : Agence Nationale de la Recherche
ID : ANR-16-C11-0017-01
Organisme : SPring-8 Proposal
ID : 2016B1182
Organisme : SPring-8 Proposal
ID : 2019B1365
Informations de copyright
© 2020, Estévez-Gallego et al.
Déclaration de conflit d'intérêts
JE, FJ, SK, RB, FB, AP, DL, CK, TY, HI, LD, IB, MS, DC, SK, JD, MO No competing interests declared
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