The HARE chip for efficient time-resolved serial synchrotron crystallography.
HARE
LAMA
data collection
fixed-target serial synchrotron crystallography
sample delivery
time-resolved crystallography
Journal
Journal of synchrotron radiation
ISSN: 1600-5775
Titre abrégé: J Synchrotron Radiat
Pays: United States
ID NLM: 9888878
Informations de publication
Date de publication:
01 Mar 2020
01 Mar 2020
Historique:
received:
19
11
2019
accepted:
20
01
2020
entrez:
11
3
2020
pubmed:
11
3
2020
medline:
11
3
2020
Statut:
ppublish
Résumé
Serial synchrotron crystallography (SSX) is an emerging technique for static and time-resolved protein structure determination. Using specifically patterned silicon chips for sample delivery, the `hit-and-return' (HARE) protocol allows for efficient time-resolved data collection. The specific pattern of the crystal wells in the HARE chip provides direct access to many discrete time points. HARE chips allow for optical excitation as well as on-chip mixing for reaction initiation, making a large number of protein systems amenable to time-resolved studies. Loading of protein microcrystals onto the HARE chip is streamlined by a novel vacuum loading platform that allows fine-tuning of suction strength while maintaining a humid environment to prevent crystal dehydration. To enable the widespread use of time-resolved serial synchrotron crystallography (TR-SSX), detailed technical descriptions of a set of accessories that facilitate TR-SSX workflows are provided.
Identifiants
pubmed: 32153274
pii: S1600577520000685
doi: 10.1107/S1600577520000685
pmc: PMC7064102
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
360-370Subventions
Organisme : Seventh Framework Programme, FP7 People: Marie-Curie Actions
ID : 623994
Informations de copyright
open access.
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