A lathe system for micrometre-sized cylindrical sample preparation at room and cryogenic temperatures.
Sample preparation
nano-tomography
ptychography
Journal
Journal of synchrotron radiation
ISSN: 1600-5775
Titre abrégé: J Synchrotron Radiat
Pays: United States
ID NLM: 9888878
Informations de publication
Date de publication:
01 Mar 2020
01 Mar 2020
Historique:
received:
14
10
2019
accepted:
19
12
2019
entrez:
11
3
2020
pubmed:
11
3
2020
medline:
11
3
2020
Statut:
ppublish
Résumé
A simple two-spindle based lathe system for the preparation of cylindrical samples intended for X-ray tomography is presented. The setup can operate at room temperature as well as under cryogenic conditions, allowing the preparation of samples down to 20 and 50 µm in diameter, respectively, within minutes. Case studies are presented involving the preparation of a brittle biomineral brachiopod shell and cryogenically fixed soft brain tissue, and their examination by means of ptychographic X-ray computed tomography reveals the preparation method to be mainly free from causing artefacts. Since this lathe system easily yields near-cylindrical samples ideal for tomography, a usage for a wide variety of otherwise challenging specimens is anticipated, in addition to potential use as a time- and cost-saving tool prior to focused ion-beam milling. Fast sample preparation becomes especially important in relation to shorter measurement times expected in next-generation synchrotron sources.
Identifiants
pubmed: 32153287
pii: S1600577519017028
doi: 10.1107/S1600577519017028
pmc: PMC7064112
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
472-476Subventions
Organisme : Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschungre, R'equip
ID : 145056
Organisme : Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
ID : 200021_152554
Informations de copyright
open access.
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