Splicing Characterization of

CLCNKB abnormal RNA splicing classical Bartter syndrome hypokalemia synonymous variant

Journal

Frontiers in genetics
ISSN: 1664-8021
Titre abrégé: Front Genet
Pays: Switzerland
ID NLM: 101560621

Informations de publication

Date de publication:
2020
Historique:
received: 27 09 2019
accepted: 24 01 2020
entrez: 11 3 2020
pubmed: 11 3 2020
medline: 11 3 2020
Statut: epublish

Résumé

Type III Bartter syndrome (BS) is caused by loss-of-function mutations in the gene encoding basolateral chloride channel ClC-Kb ( The genomic DNA of the four patients was screened for gene variations using whole-exome sequencing (WES). The candidate variants were validated by direct Sanger sequencing. Quantitative PCR (qPCR) was subsequently performed to confirm the whole Our patients showed early onset age with hyponatremia, hypokalemia, hypochloremia, repeated vomiting and growth retardation, suggesting Bartter syndrome. Genetic analysis revealed that all patients carried compound heterozygous or homozygous truncating variants in the Our results support that the whole

Identifiants

pubmed: 32153641
doi: 10.3389/fgene.2020.00081
pmc: PMC7047732
doi:

Types de publication

Journal Article

Langues

eng

Pagination

81

Informations de copyright

Copyright © 2020 Wang, Han, Zhou, Zheng, Zhou, Bao, Jia, Zhang, Huang, Ding and Zhao.

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Auteurs

Chunli Wang (C)

Nanjing Key Laboratory of Pediatrics, Children's Hospital of Nanjing Medical University, Nanjing, China.

Yuan Han (Y)

Department of Nephrology, Children's Hospital of Nanjing Medical University, Nanjing, China.

Jiaran Zhou (J)

Department of Nephrology, Children's Hospital of Nanjing Medical University, Nanjing, China.

Bixia Zheng (B)

Nanjing Key Laboratory of Pediatrics, Children's Hospital of Nanjing Medical University, Nanjing, China.

Wei Zhou (W)

Nanjing Key Laboratory of Pediatrics, Children's Hospital of Nanjing Medical University, Nanjing, China.

Huaying Bao (H)

Department of Nephrology, Children's Hospital of Nanjing Medical University, Nanjing, China.

Zhanjun Jia (Z)

Nanjing Key Laboratory of Pediatrics, Children's Hospital of Nanjing Medical University, Nanjing, China.

Aihua Zhang (A)

Department of Nephrology, Children's Hospital of Nanjing Medical University, Nanjing, China.
Jiangsu Key Laboratory of Pediatrics, Nanjing Medical University, Nanjing, China.

Songming Huang (S)

Department of Nephrology, Children's Hospital of Nanjing Medical University, Nanjing, China.
Jiangsu Key Laboratory of Pediatrics, Nanjing Medical University, Nanjing, China.

Guixia Ding (G)

Department of Nephrology, Children's Hospital of Nanjing Medical University, Nanjing, China.

Fei Zhao (F)

Department of Nephrology, Children's Hospital of Nanjing Medical University, Nanjing, China.

Classifications MeSH