Studying DNA Double-Strand Break Repair: An Ever-Growing Toolbox.
DNA damage
DNA repair
chromatin
homologous recombination (HR)
non-homologous DNA end joining
Journal
Frontiers in molecular biosciences
ISSN: 2296-889X
Titre abrégé: Front Mol Biosci
Pays: Switzerland
ID NLM: 101653173
Informations de publication
Date de publication:
2020
2020
Historique:
received:
15
11
2019
accepted:
04
02
2020
entrez:
11
3
2020
pubmed:
11
3
2020
medline:
11
3
2020
Statut:
epublish
Résumé
To ward off against the catastrophic consequences of persistent DNA double-strand breaks (DSBs), eukaryotic cells have developed a set of complex signaling networks that detect these DNA lesions, orchestrate cell cycle checkpoints and ultimately lead to their repair. Collectively, these signaling networks comprise the DNA damage response (DDR). The current knowledge of the molecular determinants and mechanistic details of the DDR owes greatly to the continuous development of ground-breaking experimental tools that couple the controlled induction of DSBs at distinct genomic positions with assays and reporters to investigate DNA repair pathways, their impact on other DNA-templated processes and the specific contribution of the chromatin environment. In this review, we present these tools, discuss their pros and cons and illustrate their contribution to our current understanding of the DDR.
Identifiants
pubmed: 32154266
doi: 10.3389/fmolb.2020.00024
pmc: PMC7047327
doi:
Types de publication
Journal Article
Review
Langues
eng
Pagination
24Informations de copyright
Copyright © 2020 Vítor, Huertas, Legube and de Almeida.
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