Structure of an ancestral mammalian family 1B1 cytochrome P450 with increased thermostability.

Amino Acid Sequence Animals Benzoflavones / metabolism Crystallography, X-Ray Cytochrome P-450 CYP1B1 / chemistry Enzyme Stability Estradiol / metabolism Humans Models, Molecular Protein Binding Protein Conformation Temperature
16beta-estradiol CYP1B1 X-ray crystallography alpha-naphthoflavone ancestral reconstruction cytochrome P450 drug metabolism enzyme stability enzyme structure estradiol human membrane protein protein engineering protein structure reconstructed ancestral protein synthetic biology thermostability

Journal

The Journal of biological chemistry
ISSN: 1083-351X
Titre abrégé: J Biol Chem
Pays: United States
ID NLM: 2985121R

Informations de publication

Date de publication:
24 04 2020
Historique:
received: 04 09 2019
revised: 09 03 2020
pubmed: 12 3 2020
medline: 22 12 2020
entrez: 12 3 2020
Statut: ppublish

Résumé

Mammalian cytochrome P450 enzymes often metabolize many pharmaceuticals and other xenobiotics, a feature that is valuable in a biotechnology setting. However, extant P450 enzymes are typically relatively unstable, with

Identifiants

DOI: 10.1074/jbc.RA119.010727 PMID: 32156703 PMC: PMC7186169
pubmed: 32156703
pii: S0021-9258(17)50294-X
doi: 10.1074/jbc.RA119.010727
pmc: PMC7186169
pii:
doi:

Substances chimiques

Benzoflavones 0
Estradiol 4TI98Z838E
alpha-naphthoflavone 604-59-1
CYP1B1 protein, human EC 1.14.14.1
Cytochrome P-450 CYP1B1 EC 1.14.14.1

Banques de données

PDB
['3PM0', '6OYU']

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

5640-5653

Subventions

Organisme : NIGMS NIH HHS
ID : R37 GM076343
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM008545
Pays : United States

Informations de copyright

© 2020 Bart et al.

Références

Drug Metab Dispos. 1997 May;25(5):617-22
pubmed: 9152602
Curr Opin Biotechnol. 2011 Dec;22(6):809-17
pubmed: 21411308
Drug Metab Dispos. 2005 Apr;33(4):489-94
pubmed: 15616152
Methods Enzymol. 1997;276:307-26
pubmed: 27754618
J Inorg Biochem. 2004 Jul;98(7):1194-9
pubmed: 15219985
J Biol Chem. 2000 Oct 6;275(40):31086-92
pubmed: 10859321
J Biomol Screen. 2008 Feb;13(2):135-41
pubmed: 18216397
Angew Chem Int Ed Engl. 2007;46(44):8414-8
pubmed: 17886313
Annu Rev Pharmacol Toxicol. 2001;41:297-316
pubmed: 11264459
Chem Res Toxicol. 1998 Sep;11(9):1048-56
pubmed: 9760279
Acta Crystallogr D Biol Crystallogr. 1994 Mar 1;50(Pt 2):178-85
pubmed: 15299456
Mol Pharmacol. 2008 Jun;73(6):1844-56
pubmed: 18372398
Acta Crystallogr D Biol Crystallogr. 2010 Jan;66(Pt 1):22-5
pubmed: 20057045
Chem Res Toxicol. 2009 Jul;22(7):1325-33
pubmed: 19563207
Biochem Pharmacol. 2000 Jan 1;59(1):65-85
pubmed: 10605936
Hum Mol Genet. 1997 Apr;6(4):641-7
pubmed: 9097971
Biochim Biophys Acta Proteins Proteom. 2018 Jan;1866(1):97-115
pubmed: 28822812
J Biol Chem. 2013 May 3;288(18):12932-43
pubmed: 23508959
J Biol Chem. 1964 Jul;239:2370-8
pubmed: 14209971
Cancer Res. 1996 Jul 1;56(13):2979-84
pubmed: 8674051
Metab Eng. 2011 Nov;13(6):682-93
pubmed: 21963485
Cancer Res. 1997 Jul 15;57(14):3026-31
pubmed: 9230218
Acta Crystallogr D Biol Crystallogr. 2009 Oct;65(Pt 10):1074-80
pubmed: 19770504
Chem Res Toxicol. 1999 Jul;12(7):623-9
pubmed: 10409402
Nat Biotechnol. 2002 Nov;20(11):1135-9
pubmed: 12368811
Arch Biochem Biophys. 1993 Aug 15;305(1):123-31
pubmed: 8342945
Chem Res Toxicol. 2002 May;15(5):614-22
pubmed: 12018981
J Biol Chem. 2007 May 11;282(19):14348-55
pubmed: 17311915
Biochemistry. 2008 Feb 19;47(7):2071-9
pubmed: 18197710
PLoS One. 2011;6(12):e28257
pubmed: 22164255
J Biol Chem. 2018 Dec 14;293(50):19201-19210
pubmed: 30254074
Appl Environ Microbiol. 1998 May;64(5):1694-9
pubmed: 9572938
Int J Tryptophan Res. 2015 Feb 10;8:7-18
pubmed: 25733915
Mol Biol Evol. 2007 Dec;24(12):2619-31
pubmed: 17916789
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501
pubmed: 20383002
Toxicol Pathol. 2004 Nov-Dec;32(6):710-8
pubmed: 15580705
Proc Natl Acad Sci U S A. 2001 Aug 28;98(18):10037-41
pubmed: 11517324
Biochim Biophys Acta. 1991 Jun 19;1084(1):35-40
pubmed: 1905157
Annu Rev Pharmacol Toxicol. 2008;48:333-58
pubmed: 17914928
Mol Pharmacol. 2013 Sep;84(3):451-8
pubmed: 23821647
Mutagenesis. 1997 Mar;12(2):83-9
pubmed: 9106248
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):213-21
pubmed: 20124702
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):125-32
pubmed: 20124692
Chem Res Toxicol. 1995 Jan-Feb;8(1):136-42
pubmed: 7703357
Drug Metab Rev. 2016 Aug;48(3):342-50
pubmed: 27358009
J Biol Chem. 2011 Feb 18;286(7):5736-43
pubmed: 21147782
Acta Crystallogr D Biol Crystallogr. 2013 Jul;69(Pt 7):1204-14
pubmed: 23793146
J Mol Evol. 2006 Jun;62(6):708-17
pubmed: 16752211
Mamm Genome. 2010 Jun;21(5-6):320-9
pubmed: 20495808
J Appl Crystallogr. 2007 Aug 1;40(Pt 4):658-674
pubmed: 19461840
Arch Biochem Biophys. 1998 Sep 1;357(1):111-20
pubmed: 9721189
Drug Metab Dispos. 2004 Aug;32(8):840-7
pubmed: 15258110
Mutat Res. 2001 May 31;492(1-2):81-90
pubmed: 11377247
Proc Natl Acad Sci U S A. 1996 Sep 3;93(18):9776-81
pubmed: 8790407
FEBS Lett. 1995 Oct 30;374(2):270-2
pubmed: 7589551
J Biol Chem. 2003 Jan 3;278(1):608-16
pubmed: 12401810

Auteurs

Aaron G Bart (AG)

Program in Biophysics, University of Michigan, Ann Arbor, Michigan 48109.

Kurt L Harris (KL)

School of Chemistry and Molecular Biosciences, University of Queensland St. Lucia, Brisbane 4072, Australia.

Elizabeth M J Gillam (EMJ)

School of Chemistry and Molecular Biosciences, University of Queensland St. Lucia, Brisbane 4072, Australia.

Emily E Scott (EE)

Program in Biophysics, University of Michigan, Ann Arbor, Michigan 48109; Departments of Medicinal Chemistry and Pharmacology, University of Michigan, Ann Arbor, Michigan 48109. Electronic address: scottee@umich.edu.

Articles similaires

[Redispensing of expensive oral anticancer medicines: a practical application].

Lisanne N van Merendonk, Kübra Akgöl, Bastiaan Nuijen
1.00
Humans Antineoplastic Agents Administration, Oral Drug Costs Counterfeit Drugs

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female

Evaluation of Low-Value Services Across Major Medicare Advantage Insurers and Traditional Medicare.

Ciara Duggan, Adam L Beckman, Ishani Ganguli et al.
1.00
Humans United States Aged Cross-Sectional Studies Medicare Part C

Effectiveness of Virtual Yoga for Chronic Low Back Pain: A Randomized Clinical Trial.

Hallie Tankha, Devyn Gaskins, Amanda Shallcross et al.
1.00
Humans Yoga Low Back Pain Female Male

Classifications MeSH

questionsmedicales.fr › Sciences de l'information › Sources d'information › Services d'information › Documentation › Données de séquences moléculaires › Séquence d'acides aminés › Structure of an ancestral mammalian family 1B1...
questionsmedicales.fr › Eucaryotes › Animaux › Structure of an ancestral mammalian family 1B1...
questionsmedicales.fr › Composés hétérocycliques › Composés hétérocycliques à cycles fusionnés › Composés hétérobicycliques › Benzopyranes › 4H-1-Benzopyran-4-ones › Flavonoïdes › Naphtoflavones › Structure of an ancestral mammalian family 1B1...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Cristallographie aux rayons X › Structure of an ancestral mammalian family 1B1...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Hémoprotéines › Cytochromes › Cytochrome P-450 enzyme system › Famille-1 de cytochromes P450 › Cytochrome P-450 CYP1B1 › Structure of an ancestral mammalian family 1B1...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Stabilité protéique › Stabilité enzymatique › Structure of an ancestral mammalian family 1B1...
questionsmedicales.fr › Hormones gonadiques › Hormones sexuelles stéroïdiennes › Congénères de l'oestradiol › Oestradiol › Structure of an ancestral mammalian family 1B1...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Structure of an ancestral mammalian family 1B1...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles moléculaires › Structure of an ancestral mammalian family 1B1...
questionsmedicales.fr › Métabolisme › Liaison aux protéines › Structure of an ancestral mammalian family 1B1...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Structure of an ancestral mammalian family 1B1...
questionsmedicales.fr › Environnement et santé publique › Environnement › Concepts météorologiques › Atmosphère › Temps (météorologie) › Température › Structure of an ancestral mammalian family 1B1...