Effects of C-Terminal Domain of the Heavy Chain of Tetanus Toxin on Gut Microbiota in a Rat Model of Depression.

Journal

Clinical pharmacology and translational medicine

ISSN: 2572-7656

Titre abrégé: Clin Pharmacol Transl Med

Pays: United States

ID NLM: 101718870

Informations de publication

Date de publication:
2019

Historique:

entrez: 12 3 2020

pubmed: 1 1 2019

medline: 1 1 2019

Statut: ppublish

Résumé

It is now well established that imbalance or dysbiosis in the gut microbiota (GM) plays a significant role in neuropsychiatric/neurodegenerative disorders. Recently it has been reported that the C-terminal domain of the heavy chain of tetanus toxin (Hc-TeTx) may not only act as a neuroprotectant but may also exhibit antidepressant effects in Wistar-Kyoto (WKY) rats, a putative animal model of treatment-resistant depression. The aim of this study was to determine whether Hc-TeTx may also interact with GM implicated in mood regulation in these rats. Adult male WKY rats (5/group) were injected intramuscularly (IM) with 60 μg/kg Hc-TeTx or saline. Twenty-four hours after the injection, the animals were sacrificed, intestinal stools were collected and stored at -80°C. DNA was extracted from the samples for 16S rRNA gene-based microbiota analysis using 16S Metagenomics application. Abundance of several bacteria at different taxonomic levels were distinguished between Hc-TeTx group and the control. At species-level, 11 operational taxonomic units (OTUs), particularly Hc-TeTx enhanced probiotic species and suppressed the opportunistic pathogens. Since overall effect of Hc-TeTx appears to be promoting GM associated with mood enhancement (e.g.

Sections du résumé

BACKGROUND/AIMS OBJECTIVE

METHODS METHODS

Adult male WKY rats (5/group) were injected intramuscularly (IM) with 60 μg/kg Hc-TeTx or saline. Twenty-four hours after the injection, the animals were sacrificed, intestinal stools were collected and stored at -80°C. DNA was extracted from the samples for 16S rRNA gene-based microbiota analysis using 16S Metagenomics application.

RESULTS RESULTS

Abundance of several bacteria at different taxonomic levels were distinguished between Hc-TeTx group and the control. At species-level, 11 operational taxonomic units (OTUs), particularly

CONCLUSION CONCLUSIONS

Hc-TeTx enhanced probiotic species and suppressed the opportunistic pathogens. Since overall effect of Hc-TeTx appears to be promoting GM associated with mood enhancement (e.g.

Identifiants

PMID: 32159077 PMC: PMC7063687

pubmed: 32159077

pmc: PMC7063687

mid: NIHMS1065711

Types de publication

Journal Article

Langues

eng

Pagination

152-159

Subventions

Organisme : NIAAA NIH HHS

ID : R03 AA022479

Pays : United States

Déclaration de conflit d'intérêts

Conflict of Interest The authors state that they have no conflict of interest.

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