Cellular Classes in the Human Brain Revealed In Vivo by Heartbeat-Related Modulation of the Extracellular Action Potential Waveform.
LFP coupling
biophysical modeling
cardiovascular
cell types
heartbeat
hippocampus
human recordings
morphology
slice physiology
spike waveform
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
10 03 2020
10 03 2020
Historique:
received:
09
09
2019
revised:
23
12
2019
accepted:
05
02
2020
entrez:
12
3
2020
pubmed:
12
3
2020
medline:
24
3
2021
Statut:
ppublish
Résumé
Determining cell types is critical for understanding neural circuits but remains elusive in the living human brain. Current approaches discriminate units into putative cell classes using features of the extracellular action potential (EAP); in absence of ground truth data, this remains a problematic procedure. We find that EAPs in deep structures of the brain exhibit robust and systematic variability during the cardiac cycle. These cardiac-related features refine neural classification. We use these features to link bio-realistic models generated from in vitro human whole-cell recordings of morphologically classified neurons to in vivo recordings. We differentiate aspiny inhibitory and spiny excitatory human hippocampal neurons and, in a second stage, demonstrate that cardiac-motion features reveal two types of spiny neurons with distinct intrinsic electrophysiological properties and phase-locking characteristics to endogenous oscillations. This multi-modal approach markedly improves cell classification in humans, offers interpretable cell classes, and is applicable to other brain areas and species.
Identifiants
pubmed: 32160555
pii: S2211-1247(20)30188-1
doi: 10.1016/j.celrep.2020.02.027
pmc: PMC7159630
mid: NIHMS1574780
pii:
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
3536-3551.e6Subventions
Organisme : NIMH NIH HHS
ID : P50 MH094258
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH110831
Pays : United States
Organisme : NINDS NIH HHS
ID : U01 NS098961
Pays : United States
Organisme : NINDS NIH HHS
ID : U01 NS103792
Pays : United States
Informations de copyright
Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests.
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