Silver-Containing Titanium Dioxide Nanocapsules for Combating Multidrug-Resistant Bacteria.
Animals
Anti-Bacterial Agents
/ chemistry
Coated Materials, Biocompatible
/ chemistry
Drug Resistance, Multiple, Bacterial
/ drug effects
Escherichia coli
/ drug effects
Macrophages
/ drug effects
Mice, Inbred C57BL
Microbial Sensitivity Tests
Nanocapsules
/ chemistry
Silver
/ chemistry
Staphylococcus aureus
/ drug effects
Titanium
/ chemistry
antimicrobial effect
immune cell uptake
multidrug-resistant S. aureus
silver nanoparticles
titanium dioxide nanocapsules
Journal
International journal of nanomedicine
ISSN: 1178-2013
Titre abrégé: Int J Nanomedicine
Pays: New Zealand
ID NLM: 101263847
Informations de publication
Date de publication:
2020
2020
Historique:
received:
21
09
2019
accepted:
24
12
2019
entrez:
13
3
2020
pubmed:
13
3
2020
medline:
20
6
2020
Statut:
epublish
Résumé
Joint arthroplasty has improved the quality of life of patients worldwide, but infections of the prosthesis are frequent and cause significant morbidity. Antimicrobial coatings for implants promise to prevent these infections. We have synthesized nanocapsules of titanium dioxide in amorphous or anatase form containing silver as antibacterial agent and tested their impact on bacterial growth. Furthermore, we explored the possible effect of the nanocapsules on the immune system. First, we studied their uptake into macrophages using a combination of electron microscopy and energy-dispersive spectroscopy. Second, we exposed immune cells to the nanocapsules and checked their activation state by flow cytometry and enzyme-linked immunosorbent assay. Silver-containing titanium dioxide nanocapsules show strong antimicrobial activity against both Our bactericidal silver-containing titanium dioxide nanocapsules fulfill important prerequisites for biomedical use and represent a promising material for the coating of artificial implants.
Sections du résumé
BACKGROUND
BACKGROUND
Joint arthroplasty has improved the quality of life of patients worldwide, but infections of the prosthesis are frequent and cause significant morbidity. Antimicrobial coatings for implants promise to prevent these infections.
METHODS
METHODS
We have synthesized nanocapsules of titanium dioxide in amorphous or anatase form containing silver as antibacterial agent and tested their impact on bacterial growth. Furthermore, we explored the possible effect of the nanocapsules on the immune system. First, we studied their uptake into macrophages using a combination of electron microscopy and energy-dispersive spectroscopy. Second, we exposed immune cells to the nanocapsules and checked their activation state by flow cytometry and enzyme-linked immunosorbent assay.
RESULTS
RESULTS
Silver-containing titanium dioxide nanocapsules show strong antimicrobial activity against both
CONCLUSION
CONCLUSIONS
Our bactericidal silver-containing titanium dioxide nanocapsules fulfill important prerequisites for biomedical use and represent a promising material for the coating of artificial implants.
Identifiants
pubmed: 32161457
doi: 10.2147/IJN.S231949
pii: 231949
pmc: PMC7050041
doi:
Substances chimiques
Anti-Bacterial Agents
0
Coated Materials, Biocompatible
0
Nanocapsules
0
titanium dioxide
15FIX9V2JP
Silver
3M4G523W1G
Titanium
D1JT611TNE
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1267-1281Informations de copyright
© 2020 Hérault et al.
Déclaration de conflit d'intérêts
The authors report no conflicts of interest in this work.
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