Discordant Activity of Kaempferol Towards Dengue Virus and Japanese Encephalitis Virus.
Animals
Cell Line
Cricetinae
Dengue
/ drug therapy
Dengue Virus
/ drug effects
Encephalitis Virus, Japanese
/ drug effects
Encephalitis, Japanese
/ drug therapy
Endoplasmic Reticulum Chaperone BiP
HEK293 Cells
Heat-Shock Proteins
/ genetics
Humans
Kaempferols
/ pharmacology
Protein Biosynthesis
/ drug effects
Transcription, Genetic
/ drug effects
Virus Replication
/ drug effects
Japanese encephalitis virus
Kaempferol
antiviral activity
dengue virus
Journal
Molecules (Basel, Switzerland)
ISSN: 1420-3049
Titre abrégé: Molecules
Pays: Switzerland
ID NLM: 100964009
Informations de publication
Date de publication:
10 Mar 2020
10 Mar 2020
Historique:
received:
22
02
2020
revised:
06
03
2020
accepted:
08
03
2020
entrez:
14
3
2020
pubmed:
14
3
2020
medline:
15
12
2020
Statut:
epublish
Résumé
Kaempferol, a plant-derived flavonoid, has been reported to have activity against Japanese encephalitis virus (JEV) in BHK-21 cells. To determine the broader utility of this compound, we initially evaluated the activity of kaempferol against JEV and dengue virus (DENV) in HEK293T/17 cells. Results showed no significant antiviral activity against either virus. We subsequently investigated the activity of kaempferol against both JEV and DENV in BHK-21 cells. Results showed a significant inhibition of JEV infection but, surprisingly, a significant enhancement of DENV infection. The effect of kaempferol on both host protein expression and transcription was investigated and both transcriptional and translational inhibitory effects were observed, although a more marked effect was observed on host cell protein expression. Markedly, while GRP78 was increased in DENV infected cells treated with kaempferol, it was not increased in JEV infected cells treated with kaempferol. These results show that cellular alteration induced by one compound can have opposite effects on viruses from the same family, suggesting the presence of distinct replication strategies for these two viruses.
Identifiants
pubmed: 32164193
pii: molecules25051246
doi: 10.3390/molecules25051246
pmc: PMC7179415
pii:
doi:
Substances chimiques
Endoplasmic Reticulum Chaperone BiP
0
HSPA5 protein, human
0
Heat-Shock Proteins
0
Kaempferols
0
kaempferol
731P2LE49E
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Thailand Research Fund
ID : BRG6080006
Organisme : Thailand Research Fund
ID : IRN58W0002
Organisme : Mahidol University
ID : A46/2562
Organisme : Thailand Research Fund
ID : IRN5802PHDW04
Organisme : Thailand Graduate Institute of Science and Technology
ID : TG-22-14-59-005D
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