Acid ceramidase of macrophages traps herpes simplex virus in multivesicular bodies and protects from severe disease.

Acid Ceramidase / genetics Animals Female Herpes Simplex / enzymology Herpesvirus 1, Human / physiology Humans Macrophages / enzymology Mice Mice, Inbred C57BL Mice, Knockout Multivesicular Bodies / virology Virus Replication

Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
12 03 2020
Historique:
received: 25 07 2019
accepted: 17 02 2020
entrez: 14 3 2020
pubmed: 14 3 2020
medline: 28 7 2020
Statut: epublish

Résumé

Macrophages have important protective functions during infection with herpes simplex virus type 1 (HSV-1). However, molecular mechanisms that restrict viral propagation and protect from severe disease are unclear. Here we show that macrophages take up HSV-1 via endocytosis and transport the virions into multivesicular bodies (MVBs). In MVBs, acid ceramidase (aCDase) converts ceramide into sphingosine and increases the formation of sphingosine-rich intraluminal vesicles (ILVs). Once HSV-1 particles reach MVBs, sphingosine-rich ILVs bind to HSV-1 particles, which restricts fusion with the limiting endosomal membrane and prevents cellular infection. Lack of aCDase in macrophage cultures or in Asah1

Identifiants

DOI: 10.1038/s41467-020-15072-8 PMID: 32165633 PMC: PMC7067866
pubmed: 32165633
doi: 10.1038/s41467-020-15072-8
pii: 10.1038/s41467-020-15072-8
pmc: PMC7067866
doi:

Substances chimiques

Acid Ceramidase EC 3.5.1.23

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1338

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Auteurs

Judith Lang (J)

Institute of Immunology, University of Duisburg-Essen, Hufelandstr. 55, Essen, D-45147, Germany.
ORCID: 0000-0001-5352-106X

Patrick Bohn (P)

Institute of Immunology, University of Duisburg-Essen, Hufelandstr. 55, Essen, D-45147, Germany.

Hilal Bhat (H)

Institute of Immunology, University of Duisburg-Essen, Hufelandstr. 55, Essen, D-45147, Germany.

Holger Jastrow (H)

Institute of Anatomy, University of Duisburg-Essen, Hufelandstr. 55, Essen, D-45147, Germany.
Institut for Experimental Immunology and Imaging, Imaging Center Essen, Electron Microscopy Unit, University of Duisburg-Essen, Hufelandstr. 55, Essen, D-45147, Germany.

Bernd Walkenfort (B)

Institut for Experimental Immunology and Imaging, Imaging Center Essen, Electron Microscopy Unit, University of Duisburg-Essen, Hufelandstr. 55, Essen, D-45147, Germany.

Feyza Cansiz (F)

Institute of Immunology, University of Duisburg-Essen, Hufelandstr. 55, Essen, D-45147, Germany.

Julian Fink (J)

Institute of Organic Chemistry, Julius-Maximilians University of Würzburg, Am Hubland, Würzburg, D-97074, Germany.

Michael Bauer (M)

Department of Molecular Life Sciences, University of Zurich, Winterthurerstr. 190, CH-8057, Zurich, Switzerland.
ORCID: 0000-0002-8156-4603

Dominik Olszewski (D)

Department of Molecular Life Sciences, University of Zurich, Winterthurerstr. 190, CH-8057, Zurich, Switzerland.

Ana Ramos-Nascimento (A)

Institute of Virology, Hannover Medical School, Carl-Neuberg-Str. 1, Hannover, D-30625, Germany.

Vikas Duhan (V)

Institute of Immunology, University of Duisburg-Essen, Hufelandstr. 55, Essen, D-45147, Germany.

Sarah-Kim Friedrich (SK)

Institute of Immunology, University of Duisburg-Essen, Hufelandstr. 55, Essen, D-45147, Germany.

Katrin Anne Becker (KA)

Institute of Molecular Biology, University of Duisburg-Essen, Hufelandstr. 55, Essen, D-45147, Germany.

Adalbert Krawczyk (A)

Institute for Virology, University of Duisburg-Essen, Hufelandstr. 55, Essen, D-45147, Germany.
Department of Infectious Diseases, University Hospital of Essen, University of Duisburg-Essen, Hufelandstr. 55, Essen, D-45147, Germany.

Michael J Edwards (MJ)

Department of Surgery, University of Cincinnati, Cincinnati, OH, USA.

Andreas Burchert (A)

Department of Hematology, Oncology and Immunology, University Hospital Giessen and Marburg, Campus Marburg, Baldingerstr., Marburg, D-35043, Germany.

Magdalena Huber (M)

Institute of Medical Microbiology and Hospital Hygiene, Philipps-University Marburg, Hans-Meerwein Str. 2, Marburg, D-35043, Germany.

Justa Friebus-Kardash (J)

Institute of Immunology, University of Duisburg-Essen, Hufelandstr. 55, Essen, D-45147, Germany.

Joachim R Göthert (JR)

Department of Hematology, West German Cancer Center, University Hospital of Essen, University of Duisburg-Essen, Hufelandstr. 55, Essen, D-45147, Germany.

Cornelia Hardt (C)

Institute of Immunology, University of Duisburg-Essen, Hufelandstr. 55, Essen, D-45147, Germany.

Hans Christian Probst (HC)

Institute of Immunology, University Medical Center Mainz, Langenbeckstr. 1, Mainz, D-55131, Germany.
ORCID: 0000-0002-7926-0651

Fabian Schumacher (F)

Institute of Molecular Biology, University of Duisburg-Essen, Hufelandstr. 55, Essen, D-45147, Germany.
Institute of Nutritional Science, University of Potsdam, Arthur-Scheunert Allee 114-116, Nuthetal, D-14558, Germany.
ORCID: 0000-0001-8703-3275

Karl Köhrer (K)

Biological and Medical Research Center (BMFZ), Heinrich-Heine-University, Universitätsstr. 1, Düsseldorf, D-40225, Germany.
ORCID: 0000-0003-3644-2022

Burkhard Kleuser (B)

Institute of Nutritional Science, University of Potsdam, Arthur-Scheunert Allee 114-116, Nuthetal, D-14558, Germany.

Eduard B Babiychuk (EB)

Institute of Anatomy, University of Bern, Baltzerstr. 4, CH-3012, Bern, Switzerland.

Beate Sodeik (B)

Institute of Virology, Hannover Medical School, Carl-Neuberg-Str. 1, Hannover, D-30625, Germany.
Cluster of Excellence RESIST (EXC 2155), Hannover Medical School, Carl-Neuberg-Str. 1, Hannover, D-30625, Germany.
ORCID: 0000-0003-4650-3036

Jürgen Seibel (J)

Institute of Organic Chemistry, Julius-Maximilians University of Würzburg, Am Hubland, Würzburg, D-97074, Germany.

Urs F Greber (UF)

Department of Molecular Life Sciences, University of Zurich, Winterthurerstr. 190, CH-8057, Zurich, Switzerland.
ORCID: 0000-0003-2278-120X

Philipp A Lang (PA)

Department of Molecular Medicine II, Medical Faculty, Heinrich Heine University, Universitätsstr. 1, Düsseldorf, D-40225, Germany.

Erich Gulbins (E)

Institute of Molecular Biology, University of Duisburg-Essen, Hufelandstr. 55, Essen, D-45147, Germany.
Department of Surgery, University of Cincinnati, Cincinnati, OH, USA.
ORCID: 0000-0002-3117-1342

Karl S Lang (KS)

Institute of Immunology, University of Duisburg-Essen, Hufelandstr. 55, Essen, D-45147, Germany. karlsebastian.lang@uk-essen.de.

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Classifications MeSH

questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Amidohydrolases › Ceramidases › Acid Ceramidase › Acid ceramidase of macrophages traps herpes...
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