Rapid diagnosis of periodontitis, a feasibility study using MALDI-TOF mass spectrometry.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
07
11
2019
accepted:
26
02
2020
entrez:
14
3
2020
pubmed:
14
3
2020
medline:
27
6
2020
Statut:
epublish
Résumé
The aim of the present study was to assess the feasibility and diagnostic contribution of protein profiling using MALDI-TOF mass spectrometry applied to saliva, gingival crevicular fluid (GCF) and dental plaque from periodontitis and healthy subjects. We hypothesized that rapid routine and blinded MALDI-TOF analysis could accurately classify these three types of samples according to periodontal state. Unstimulated saliva, GCF and dental plaque, collected from periodontitis subjects and healthy controls, were analyzed by MALDI-TOF MS. Based on the differentially expressed peaks between the two groups, diagnostic decision trees were built for each sample. Among 141 patients (67 periodontitis and 74 healthy controls), the decision trees diagnosed periodontitis with a sensitivity = 70.3% (± 0.211) and a specificity = 77.8% (± 0.165) for saliva, a sensitivity = 79.6% (± 0.188) and a specificity = 75.7% (± 0.195) for GCF, and a sensitivity = 72.1% (± 0.202) and a specificity = 72.2% (± 0.195) for dental plaque. The sensitivity and specificity of the tests were improved to 100% (CI 95% = [0.91;1]) and 100% (CI 95% = [0.92;1]), respectively, when two samples were tested. We developed, for the first time, diagnostic tests based on protein profiles of saliva, GCF and dental plaque between periodontitis patients and healthy subjects. When at least 2 of these samples were tested, the best results were obtained.
Identifiants
pubmed: 32168352
doi: 10.1371/journal.pone.0230334
pii: PONE-D-19-31089
pmc: PMC7069628
doi:
Substances chimiques
Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0230334Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
PLoS One. 2011;6(12):e28182
pubmed: 22164240
Anal Bioanal Chem. 2019 Nov;411(28):7551-7562
pubmed: 31641822
Proteomics Clin Appl. 2014 Oct;8(9-10):748-61
pubmed: 24828325
Mass Spectrom Rev. 2013 May-Jun;32(3):188-217
pubmed: 22996584
Bioinformatics. 2012 Sep 1;28(17):2270-1
pubmed: 22796955
Proteome Sci. 2012 Nov 06;10(1):63
pubmed: 23126675
PLoS Negl Trop Dis. 2017 Jul 24;11(7):e0005762
pubmed: 28742123
J Evid Based Dent Pract. 2012 Sep;12(3 Suppl):20-8
pubmed: 23040337
Oral Dis. 2013 Sep;19(6):585-91
pubmed: 23170808
J Clin Periodontol. 2015 Apr;42 Suppl 16:S59-70
pubmed: 25496279
PLoS One. 2014 Dec 05;9(12):e114430
pubmed: 25478864
Biomed Res Int. 2019 Feb 5;2019:7850392
pubmed: 30868074
Bioinformatics. 2015 Oct 1;31(19):3156-62
pubmed: 26026136
Nat Protoc. 2009;4(5):732-42
pubmed: 19390529
J Periodontal Res. 2013 Jun;48(3):331-41
pubmed: 23050757
J Clin Virol. 2018 May;102:77-83
pubmed: 29525634
Proteomics. 2016 Mar;16(6):1033-45
pubmed: 26711623
PLoS One. 2009 Nov 25;4(11):e8041
pubmed: 19946369
J Oral Microbiol. 2019 Aug 26;11(1):1654786
pubmed: 31497257
PLoS One. 2018 Dec 20;13(12):e0200231
pubmed: 30571680
Clin Chem. 2007 May;53(5):852-8
pubmed: 17395711
J Proteome Res. 2010 Apr 5;9(4):1683-93
pubmed: 20020772
Arch Oral Biol. 2016 Dec;72:92-98
pubmed: 27565354
Future Microbiol. 2010 Nov;5(11):1733-54
pubmed: 21133692
PLoS One. 2015 May 20;10(5):e0127660
pubmed: 25993052
J Periodontol. 2018 Jun;89 Suppl 1:S173-S182
pubmed: 29926951
Arch Oral Biol. 2018 Mar;87:115-124
pubmed: 29288920
OMICS. 2010 Feb;14(1):115-26
pubmed: 20141334
Periodontol 2000. 2005;39:53-72
pubmed: 16135064
Biomed Res Int. 2018 Nov 15;2018:3130607
pubmed: 30581850
PLoS One. 2016 May 26;11(5):e0155449
pubmed: 27228175
BMC Infect Dis. 2017 Aug 31;17(1):600
pubmed: 28859607
Clin Chim Acta. 2019 Aug;495:278-286
pubmed: 31026423
PLoS One. 2017 Aug 11;12(8):e0182992
pubmed: 28800622
J Periodontol. 2015 May;86(5):611-22
pubmed: 25688694
Oral Microbiol Immunol. 2005 Oct;20(5):267-73
pubmed: 16101961
Expert Rev Mol Diagn. 2019 Feb;19(2):161-173
pubmed: 30640566
Clin Microbiol Rev. 2013 Oct;26(4):781-91
pubmed: 24092855
J Alzheimers Dis. 2017;58(2):355-359
pubmed: 28453477
J Periodontol. 2010 Jan;81(1):89-98
pubmed: 20059421
Curr Issues Mol Biol. 2017;23:17-20
pubmed: 28504240
Periodontol 2000. 2017 Oct;75(1):7-23
pubmed: 28758294
PeerJ. 2018 Feb 28;6:e4430
pubmed: 29507828
ISME J. 2018 May;12(5):1210-1224
pubmed: 29339824
Comp Immunol Microbiol Infect Dis. 2014 May;37(3):153-7
pubmed: 24878069
Clin Oral Investig. 2015 Apr;19(3):729-37
pubmed: 25078551
BMC Bioinformatics. 2008 Feb 07;9:88
pubmed: 18257918
J Periodontal Res. 2015 Oct;50(5):637-49
pubmed: 25439677
Parasitology. 2018 Aug;145(9):1170-1182
pubmed: 29409547