Mitochondrial Respiration Correlates with Prognostic Markers in Chronic Lymphocytic Leukemia and Is Normalized by Ibrutinib Treatment.

B cell receptor CCL3/CCL4 Oroboros oxygraph ZAP-70 chronic lymphocytic leukemia ibrutinib mitochondria oxygen consumption rates

Journal

Cancers
ISSN: 2072-6694
Titre abrégé: Cancers (Basel)
Pays: Switzerland
ID NLM: 101526829

Informations de publication

Date de publication:
11 Mar 2020
Historique:
received: 29 01 2020
revised: 28 02 2020
accepted: 06 03 2020
entrez: 15 3 2020
pubmed: 15 3 2020
medline: 15 3 2020
Statut: epublish

Résumé

Mitochondrial bioenergetics profiling, a measure of oxygen consumption rates, correlates with prognostic markers and can be used to assess response to therapy in chronic lymphocytic leukemia (CLL) cells. In this study, we measured mitochondrial respiration rates in primary CLL cells using respirometry to evaluate mitochondrial function. We found significant increases in mitochondrial respiration rates in CLL versus control B lymphocytes. We also observed amongst CLL patients that advanced age, female sex, zeta-chain-associated protein of 70 kD (ZAP-70

Identifiants

pubmed: 32168755
pii: cancers12030650
doi: 10.3390/cancers12030650
pmc: PMC7139649
pii:
doi:

Types de publication

Journal Article

Langues

eng

Subventions

Organisme : Leukemia and Lymphoma Society of Canada
ID : 761090638
Organisme : Cancer Care Manitoba / Research Manitoba CLL CLuster
ID : 761090863

Déclaration de conflit d'intérêts

The authors declare that there are no conflicts of interest with respect to this project. VB serves on the advisory boards of Gilead, Janssen, Astra Zeneca, and Abbvie. VB and SBG have research funding from Roche, Janssen, Abbvie, and Lundbeck. SBG has research funding from Gilead. VB also received fees from BIOGEN for patented compounds unrelated to this study. DED serves on advisory boards for Merck and AstraZeneca and has received an honorarium from Boehringer-Ingelheim.

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Auteurs

Subir Roy Chowdhury (S)

Research Institute in Oncology and Hematology, CancerCare Manitoba, Winnipeg, MB R3V 0V9, Canada.

Eric D J Bouchard (EDJ)

Research Institute in Oncology and Hematology, CancerCare Manitoba, Winnipeg, MB R3V 0V9, Canada.

Ryan Saleh (R)

Research Institute in Oncology and Hematology, CancerCare Manitoba, Winnipeg, MB R3V 0V9, Canada.

Zoann Nugent (Z)

Research Institute in Oncology and Hematology, CancerCare Manitoba, Winnipeg, MB R3V 0V9, Canada.

Cheryl Peltier (C)

Research Institute in Oncology and Hematology, CancerCare Manitoba, Winnipeg, MB R3V 0V9, Canada.

Edgard Mejia (E)

Departments of Immunology, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB R3E 0T5, Canada.

Sen Hou (S)

Departments of Immunology, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB R3E 0T5, Canada.

Carly McFall (C)

Research Institute in Oncology and Hematology, CancerCare Manitoba, Winnipeg, MB R3V 0V9, Canada.

Mandy Squires (M)

Research Institute in Oncology and Hematology, CancerCare Manitoba, Winnipeg, MB R3V 0V9, Canada.

Donna Hewitt (D)

Research Institute in Oncology and Hematology, CancerCare Manitoba, Winnipeg, MB R3V 0V9, Canada.

Linda Davidson (L)

Research Institute in Oncology and Hematology, CancerCare Manitoba, Winnipeg, MB R3V 0V9, Canada.

Garry X Shen (GX)

Departments of Internal Medicine, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB R3E 3P4, Canada.
Children's Hospital Research Institute of Manitoba, Winnipeg, MB R3E 3P4, Canada.

James B Johnston (JB)

Research Institute in Oncology and Hematology, CancerCare Manitoba, Winnipeg, MB R3V 0V9, Canada.
Departments of Internal Medicine, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB R3E 3P4, Canada.
Department of Medical Oncology and Hematology, CancerCare Manitoba, Winnipeg, MB R3E 0V9, Canada.

Christine Doucette (C)

Children's Hospital Research Institute of Manitoba, Winnipeg, MB R3E 3P4, Canada.
Departments of Physiology & Pathophysiology, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB R3E 3P4, Canada.

Grant M Hatch (GM)

Children's Hospital Research Institute of Manitoba, Winnipeg, MB R3E 3P4, Canada.
St. Boniface Hospital Albrechtsen Research Centre, Winnipeg, MB R2H 2A6, Canada.

Paul Fernyhough (P)

Departments of Pharmacology and Therapeutics, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB R3E 3P4, Canada.
Departments of Biochemistry and Medical Genetics, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB R3E 3N4, Canada.

Aaron Marshall (A)

Departments of Immunology, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB R3E 0T5, Canada.
Departments of Pharmacology and Therapeutics, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB R3E 3P4, Canada.

Spencer B Gibson (SB)

Research Institute in Oncology and Hematology, CancerCare Manitoba, Winnipeg, MB R3V 0V9, Canada.
Departments of Immunology, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB R3E 0T5, Canada.
Departments of Pharmacology and Therapeutics, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB R3E 3P4, Canada.

David E Dawe (DE)

Research Institute in Oncology and Hematology, CancerCare Manitoba, Winnipeg, MB R3V 0V9, Canada.
Departments of Internal Medicine, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB R3E 3P4, Canada.
Department of Medical Oncology and Hematology, CancerCare Manitoba, Winnipeg, MB R3E 0V9, Canada.

Versha Banerji (V)

Research Institute in Oncology and Hematology, CancerCare Manitoba, Winnipeg, MB R3V 0V9, Canada.
Departments of Internal Medicine, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB R3E 3P4, Canada.
Department of Medical Oncology and Hematology, CancerCare Manitoba, Winnipeg, MB R3E 0V9, Canada.
Departments of Pharmacology and Therapeutics, Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB R3E 3P4, Canada.

Classifications MeSH