A rapid and simple method for routine determination of antibiotic sensitivity to biofilm populations of Pseudomonas aeruginosa.
Antibiofilm
Antimicrobial susceptibility
Biofilm infections
Biofilms
Chronic bacterial infections
Pseudomonas aeruginosa
Journal
Annals of clinical microbiology and antimicrobials
ISSN: 1476-0711
Titre abrégé: Ann Clin Microbiol Antimicrob
Pays: England
ID NLM: 101152152
Informations de publication
Date de publication:
13 Mar 2020
13 Mar 2020
Historique:
received:
27
12
2019
accepted:
26
02
2020
entrez:
15
3
2020
pubmed:
15
3
2020
medline:
15
5
2020
Statut:
epublish
Résumé
Treatment of infections by Pseudomonas aeruginosa forming biofilms after antimicrobial testing on planktonic bacteria can result in substantial failure. Therefore, we offer a robust and simple experimental platform to test the impact of antimicrobials on biofilms. Antibiotic response patterns varied uniquely within biofilm formation capacity and minimal biofilm eradication concentrations (MBECs) has a significantly better discriminatory power than minimum inhibitory concentrations (MICs) to differentiate the overall efficiency of antibiotics to eradicate biofilm. Our resazurin-based 96-well-plate platform is able to emulate bacterial responses to antibiotics under biofilm conditions in a fast, simple, and cost-effective screening method adaptable to automation, and warrants trials in the clinic.
Identifiants
pubmed: 32169075
doi: 10.1186/s12941-020-00350-6
pii: 10.1186/s12941-020-00350-6
pmc: PMC7071750
doi:
Substances chimiques
Anti-Bacterial Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
8Subventions
Organisme : Chulalongkorn University
ID : No. 39 (2/61)
Organisme : Faculty of Medicine, Chulalongkorn University
ID : No. 39 (2/61)
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