Reliability of gastrointestinal barrier integrity and microbial translocation biomarkers at rest and following exertional heat stress.
endotoxin
exercise
gut
Journal
Physiological reports
ISSN: 2051-817X
Titre abrégé: Physiol Rep
Pays: United States
ID NLM: 101607800
Informations de publication
Date de publication:
03 2020
03 2020
Historique:
received:
05
12
2019
revised:
14
01
2020
accepted:
21
01
2020
entrez:
15
3
2020
pubmed:
15
3
2020
medline:
29
1
2021
Statut:
ppublish
Résumé
Exertional heat stress adversely distrupts (GI) barrier integrity and, through subsequent microbial translocation (MT), negativly impacts health. Despite widespread application, the temporal reliability of popular GI barrier integity and MT biomarkers is poorly characterised. Fourteen males completed two 80-min exertional heat stress tests (EHST) separated by 7-14 days. Venous blood was drawn pre, immediately- and 1-hr post both EHSTs. GI barrier integrity was assessed using the serum Dual-Sugar Absorption Test (DSAT), Intestinal Fatty-Acid-Binding Protein (I-FABP) and Claudin-3 (CLDN-3). MT was assessed using plasma Lipopolysaccharide Binding Protein (LBP), total 16S bacterial DNA and Bacteroides DNA. No GI barrier integrity or MT biomarker, except absolute Bacteroides DNA, displayed systematic trial order bias (p ≥ .05). I-FABP (trial 1 = Δ 0.834 ± 0.445 ng ml Each GI barrier integrity and MT translocation biomarker, except Bacteroides/total 16S ratio, had acceptable reliability at rest and postexertional heat stress.
Identifiants
pubmed: 32170836
doi: 10.14814/phy2.14374
pmc: PMC7070100
doi:
Substances chimiques
Biomarkers
0
CLDN3 protein, human
0
Claudin-3
0
Fatty Acid-Binding Proteins
0
Lactulose
4618-18-2
Rhamnose
QN34XC755A
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e14374Informations de copyright
© 2020 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.
Références
Lancet. 1986 Feb 8;1(8476):307-10
pubmed: 2868172
Nature. 2012 Jun 13;486(7402):207-14
pubmed: 22699609
J Pediatr Gastroenterol Nutr. 1995 Feb;20(2):184-8
pubmed: 7714684
Eur J Nutr. 2019 Jun;58(4):1441-1451
pubmed: 29574607
Clin Nutr. 2013 Apr;32(2):245-51
pubmed: 22892368
Expert Rev Clin Immunol. 2019 Mar;15(3):265-273
pubmed: 30601082
Cancer Causes Control. 2016 Sep;27(9):1163-6
pubmed: 27392432
J Appl Physiol. 1974 Aug;37(2):247-8
pubmed: 4850854
Appl Physiol Nutr Metab. 2017 Dec;42(12):1283-1292
pubmed: 28777927
Biochem Soc Trans. 2011 Aug;39(4):989-93
pubmed: 21787335
Shock. 2017 Jun;47(6):709-714
pubmed: 28505628
Pediatr Crit Care Med. 2015 Jan;16(1):37-44
pubmed: 25162512
Eur J Appl Physiol Occup Physiol. 1994;69(5):461-3
pubmed: 7875146
Transfusion. 2016 May;56(5):1138-47
pubmed: 26865079
Clin Biochem. 2014 Apr;47(6):471-4
pubmed: 24373926
Intern Emerg Med. 2017 Sep;12(6):821-836
pubmed: 28478489
Clin Chem. 1996 Mar;42(3):445-8
pubmed: 8598111
Physiol Rep. 2016 Oct;4(20):
pubmed: 27798350
BMC Gastroenterol. 2014 Nov 18;14:189
pubmed: 25407511
PLoS One. 2011;6(7):e22366
pubmed: 21811592
Chronobiol Int. 2005;22(2):207-25
pubmed: 16021839
Am J Physiol Regul Integr Comp Physiol. 2008 Aug;295(2):R611-23
pubmed: 18565834
Int J Sports Med. 2015 Mar;36(3):262-70
pubmed: 25525952
Sports Med. 2017 Jul;47(7):1389-1403
pubmed: 27943148
Crit Rev Biotechnol. 2017 Mar;37(2):251-261
pubmed: 26863480
Aliment Pharmacol Ther. 2017 Aug;46(3):246-265
pubmed: 28589631
Br J Sports Med. 1993 Mar;27(1):39-44
pubmed: 8457812
J Clin Gastroenterol. 2014 Mar;48(3):253-60
pubmed: 24100750
J Mol Endocrinol. 2013 Sep 11;51(2):R51-64
pubmed: 23943858
J Clin Gastroenterol. 2010 Jan;44(1):e14-9
pubmed: 19525861
J Therm Biol. 2015 Oct;53:38-45
pubmed: 26590454
Eur J Appl Physiol. 2018 Feb;118(2):389-400
pubmed: 29234915
Liver Int. 2013 Oct;33(9):1309-15
pubmed: 23763259
Gut. 2018 Sep;67(9):1716-1725
pubmed: 29934437
Ann Surg. 2010 Jun;251(6):1174-80
pubmed: 20485148
Gut Pathog. 2016 May 26;8:24
pubmed: 27239228
Eur J Appl Physiol. 2017 May;117(5):931-941
pubmed: 28290057
Med Sci Sports Exerc. 2012 Dec;44(12):2257-62
pubmed: 22776871
Antioxidants (Basel). 2018 Oct 25;7(11):
pubmed: 30366410
Med Sci Sports Exerc. 2019 Mar;51(3):436-444
pubmed: 30299412
Am J Physiol Gastrointest Liver Physiol. 2011 Mar;300(3):G477-84
pubmed: 21148400
Eur J Sport Sci. 2016 Nov;16(8):1064-72
pubmed: 26841003
J Appl Physiol. 1964 May;19:531-3
pubmed: 14173555
World J Gastroenterol. 2015 Oct 7;21(37):10609-20
pubmed: 26457021
Scand J Rehabil Med. 1970;2(2):92-8
pubmed: 5523831
Percept Mot Skills. 1986 Feb;62(1):211-20
pubmed: 3960662
Appl Physiol Nutr Metab. 2017 Sep;42(9):941-947
pubmed: 28511020
Physiol Rep. 2020 Mar;8(5):e14374
pubmed: 32170836
Am J Physiol Endocrinol Metab. 2018 Nov 1;315(5):E1062-E1074
pubmed: 30106621
Appl Physiol Nutr Metab. 2014 Sep;39(9):1070-82
pubmed: 25068884
Br J Nutr. 1974 Jul;32(1):77-97
pubmed: 4843734
Gut. 2007 Jan;56(1):61-72
pubmed: 16822808
Sports Med. 1998 Oct;26(4):217-38
pubmed: 9820922
J Sports Sci. 2017 Aug;35(16):1622-1628
pubmed: 27594321
Am J Physiol Gastrointest Liver Physiol. 2017 Mar 1;312(3):G171-G193
pubmed: 27908847
Eur J Appl Physiol. 2017 Dec;117(12):2519-2526
pubmed: 29032392
Neurogastroenterol Motil. 2012 Jun;24(6):503-12
pubmed: 22583600
Eur J Appl Physiol. 2013 Jun;113(6):1575-83
pubmed: 23314685