Propagation of human prostate tissue from induced pluripotent stem cells.
androgen receptor
differentiation
induced pluripotent stem cells
organoids
prostate
prostate cancer
stem cells
Journal
Stem cells translational medicine
ISSN: 2157-6580
Titre abrégé: Stem Cells Transl Med
Pays: England
ID NLM: 101578022
Informations de publication
Date de publication:
07 2020
07 2020
Historique:
received:
03
09
2019
revised:
10
01
2020
accepted:
29
01
2020
pubmed:
15
3
2020
medline:
22
7
2021
entrez:
15
3
2020
Statut:
ppublish
Résumé
Primary culture of human prostate organoids and patient-derived xenografts is inefficient and has limited access to clinical tissues. This hampers their use for translational study to identify new treatments. To overcome this, we established a complementary approach where rapidly proliferating and easily handled induced pluripotent stem cells enabled the generation of human prostate tissue in vivo and in vitro. By using a coculture technique with inductive urogenital sinus mesenchyme, we comprehensively recapitulated in situ 3D prostate histology, and overcame limitations in the primary culture of human prostate stem, luminal and neuroendocrine cells, as well as the stromal microenvironment. This model now unlocks new opportunities to undertake translational studies of benign and malignant prostate disease.
Identifiants
pubmed: 32170918
doi: 10.1002/sctm.19-0286
pmc: PMC7308643
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
734-745Subventions
Organisme : Medical Research Council
Pays : United Kingdom
Organisme : Cancer Research UK
ID : C9380/A25138
Pays : United Kingdom
Informations de copyright
© 2020 The Authors. STEM CELLS TRANSLATIONAL MEDICINE published by Wiley Periodicals, Inc. on behalf of AlphaMed Press.
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