Determination of ethyl glucuronide in human hair samples: Decontamination vs extraction.
alcohol biomarkers
ethyl glucuronide
external contamination
hair testing
Journal
Drug testing and analysis
ISSN: 1942-7611
Titre abrégé: Drug Test Anal
Pays: England
ID NLM: 101483449
Informations de publication
Date de publication:
Jul 2020
Jul 2020
Historique:
received:
04
02
2020
revised:
28
02
2020
accepted:
10
03
2020
pubmed:
15
3
2020
medline:
12
5
2021
entrez:
15
3
2020
Statut:
ppublish
Résumé
Decontamination of samples prior to analysis is common practice and recommended for ethyl glucuronide (EtG) hair testing. The aim of this study was to evaluate the applied decontamination procedure during routine hair EtG analysis by monitoring the ethyl glucuronide concentrations in the washing solutions from a representative cohort of individual hair samples. Hair samples from 150 individuals were tested for hair EtG by a validated routine procedure (liquid chromatography/tandem mass spectrometry). A four-step decontamination procedure (ethanol, water, acetone, dichloromethane) was applied to all samples prior to analysis. Hair samples from 20 individuals were analyzed along with the complete set of individual washing solutions. Hair samples from an additional 130 individuals were analyzed along with the corresponding aqueous wash fraction only. No EtG was detected in the washing solutions from hair samples that tested negative for EtG (n = 42). Hair samples positive for ethyl glucuronide (n = 108) were found to liberate different amounts of EtG during decontamination: whereas no, or low portions of, EtG (< 10% of extracted hair EtG) were found in the corresponding washing solutions of the majority (n = 91) of individual samples, there was a minority of samples (n = 6) with more than half of the extracted hair EtG present in the decontamination solvent. No correlation of the decontaminated amount of EtG and the extracted hair EtG was observed. Further experimental studies are necessary to investigate if the observed easily removable fraction of EtG is associated with external contamination and if analysis of wash solutions could be helpful for identifying external contamination in hair testing for ethyl glucuronide.
Substances chimiques
Glucuronates
0
ethyl glucuronide
17685-04-0
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
948-956Informations de copyright
© 2020 The Authors. Drug Testing and Analysis published by John Wiley & Sons Ltd.
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