In vitro characterization of a novel magnetic fibrin-agarose hydrogel for cartilage tissue engineering.
Biomechanical properties
Cell-biomaterial interactions
Fibrin-agarose
Hyaline cartilage
Magnetic nanoparticles
Tissue engineering
Journal
Journal of the mechanical behavior of biomedical materials
ISSN: 1878-0180
Titre abrégé: J Mech Behav Biomed Mater
Pays: Netherlands
ID NLM: 101322406
Informations de publication
Date de publication:
04 2020
04 2020
Historique:
received:
26
09
2019
revised:
04
01
2020
accepted:
04
01
2020
entrez:
17
3
2020
pubmed:
17
3
2020
medline:
15
5
2021
Statut:
ppublish
Résumé
The encapsulation of cells into biopolymer matrices enables the preparation of engineered substitute tissues. Here we report the generation of novel 3D magnetic biomaterials by encapsulation of magnetic nanoparticles and human hyaline chondrocytes within fibrin-agarose hydrogels, with potential use as articular hyaline cartilage-like tissues. By rheological measurements we observed that, (i) the incorporation of magnetic nanoparticles resulted in increased values of the storage and loss moduli for the different times of cell culture; and (ii) the incorporation of human hyaline chondrocytes into nonmagnetic and magnetic fibrin-agarose biomaterials produced a control of their swelling capacity in comparison with acellular nonmagnetic and magnetic fibrin-agarose biomaterials. Interestingly, the in vitro viability and proliferation results showed that the inclusion of magnetic nanoparticles did not affect the cytocompatibility of the biomaterials. What is more, immunohistochemistry showed that the inclusion of magnetic nanoparticles did not negatively affect the expression of type II collagen of the human hyaline chondrocytes. Summarizing, our results suggest that the generation of engineered hyaline cartilage-like tissues by using magnetic fibrin-agarose hydrogels is feasible. The resulting artificial tissues combine a stronger and stable mechanical response, with promising in vitro cytocompatibility. Further research would be required to elucidate if for longer culture times additional features typical of the extracellular matrix of cartilage could be expressed by human hyaline chondrocytes within magnetic fibrin-agarose hydrogels.
Identifiants
pubmed: 32174386
pii: S1751-6161(19)31307-4
doi: 10.1016/j.jmbbm.2020.103619
pii:
doi:
Substances chimiques
Hydrogels
0
Fibrin
9001-31-4
Sepharose
9012-36-6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
103619Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interests The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.