Dielectrophoresis assisted rapid, selective and single cell detection of antibiotic resistant bacteria with G-FETs.
Acinetobacter baumannii
/ drug effects
Anti-Bacterial Agents
/ pharmacology
Bacteria
/ drug effects
Bacterial Infections
/ drug therapy
Biosensing Techniques
/ instrumentation
Drug Resistance, Bacterial
Equipment Design
Humans
Single-Cell Analysis
/ instrumentation
Staphylococcus aureus
/ drug effects
Transistors, Electronic
Antibiotic resistance bacteria
Biosensors
Dielectrophoresis
Electrical detection
G-FET
Peptide probes
Journal
Biosensors & bioelectronics
ISSN: 1873-4235
Titre abrégé: Biosens Bioelectron
Pays: England
ID NLM: 9001289
Informations de publication
Date de publication:
15 May 2020
15 May 2020
Historique:
received:
06
12
2019
revised:
07
02
2020
accepted:
21
02
2020
pubmed:
17
3
2020
medline:
13
1
2021
entrez:
17
3
2020
Statut:
ppublish
Résumé
The rapid increase in antibiotic resistant pathogenic bacteria has become a global threat, which besides the development of new drugs, requires rapid, cheap, scalable, and accurate diagnostics. Label free biosensors relying on electrochemical, mechanical, and mass based detection of whole bacterial cells have attempted to meet these requirements. However, the trade-off between selectivity and sensitivity of such sensors remains a key challenge. In particular, point-of-care diagnostics that are able to reduce and/or prevent unneeded antibiotic prescriptions require highly specific probes with sensitive and accurate transducers that can be miniaturized and multiplexed, and that are easy to operate and cheap. Towards achieving this goal, we present a number of advances in the use of graphene field effect transistors (G-FET) including the first use of peptide probes to electrically detect antibiotic resistant bacteria in a highly specific manner. In addition, we dramatically reduce the needed concentration for detection by employing dielectrophoresis for the first time in a G-FET, allowing us to monitor changes in the Dirac point due to individual bacterial cells. Specifically, we realized rapid binding of bacterial cells to a G-FET by electrical field guiding to the device to realize an overall 3 orders of magnitude decrease in cell-concentration enabling a single-cell detection limit, and 9-fold reduction in needed time to 5 min. Utilizing our new biosensor and procedures, we demonstrate the first selective, electrical detection of the pathogenic bacterial species Staphylococcus aureus and antibiotic resistant Acinetobacter baumannii on a single platform.
Identifiants
pubmed: 32174552
pii: S0956-5663(20)30120-2
doi: 10.1016/j.bios.2020.112123
pmc: PMC7219479
mid: NIHMS1585154
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
112123Subventions
Organisme : NIAID NIH HHS
ID : R01 AI110724
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM102735
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM124231
Pays : United States
Organisme : NIAID NIH HHS
ID : U01 AI124302
Pays : United States
Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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