Biophysical Characterization of Membrane Phase Transition Profiles for the Discrimination of Outer Membrane Vesicles (OMVs) From
dynamic light scattering
gram negative bacteria
outer membrane vesicle
small-angle X-ray scattering
transmission electron microscopy
Journal
Frontiers in microbiology
ISSN: 1664-302X
Titre abrégé: Front Microbiol
Pays: Switzerland
ID NLM: 101548977
Informations de publication
Date de publication:
2020
2020
Historique:
received:
25
11
2019
accepted:
10
02
2020
entrez:
17
3
2020
pubmed:
17
3
2020
medline:
17
3
2020
Statut:
epublish
Résumé
Dynamic Light Scattering (DLS), Small Angle X-ray Scattering (SAXS) and Transmission Electron Microscopy (TEM) are physical techniques widely employed to characterize the morphology and the structure of vesicles such as liposomes or human extracellular vesicles (exosomes). Bacterial extracellular vesicles are similar in size to human exosomes, although their function and membrane properties have not been elucidated in such detail as in the case of exosomes. Here, we applied the above cited techniques, in synergy with the thermotropic characterization of the vesicles lipid membrane using a turbidimetric technique to the study of vesicles produced by Gram-negative bacteria (Outer Membrane Vesicles, OMVs) grown at different temperatures. This study demonstrated that our combined approach is useful to discriminate vesicles of different origin or coming from bacteria cultured under different experimental conditions. We envisage that in a near future the techniques employed in our work will be further implemented to discriminate complex mixtures of bacterial vesicles, thus showing great promises for biomedical or diagnostic applications.
Identifiants
pubmed: 32174900
doi: 10.3389/fmicb.2020.00290
pmc: PMC7056839
doi:
Types de publication
Journal Article
Langues
eng
Pagination
290Informations de copyright
Copyright © 2020 Sarra, Celluzzi, Bruno, Ricci, Sennato, Ortore, Casciardi, Del Chierico, Postorino, Bordi and Masotti.
Références
J Bacteriol. 1973 Dec;116(3):1273-9
pubmed: 4201772
Chem Phys Lipids. 2006 Jan;139(1):11-9
pubmed: 16253216
Infect Immun. 2012 Jun;80(6):1948-57
pubmed: 22409932
Biochim Biophys Acta. 1973 May 25;307(3):491-512
pubmed: 4581497
J Biol Chem. 1996 Mar 22;271(12):6801-9
pubmed: 8636103
Int J Mol Sci. 2017 Sep 06;18(9):
pubmed: 28878141
Biochim Biophys Acta Gen Subj. 2018 Dec;1862(12):2879-2887
pubmed: 30251702
Microbiol Mol Biol Rev. 2015 Mar;79(1):81-100
pubmed: 25652542
Nat Methods. 2007 Sep;4(9):717-9
pubmed: 17694065
Emerg Microbes Infect. 2017 Jul 26;6(7):e66
pubmed: 28745311
J Nutr. 2010 Mar;140(3):698S-712S
pubmed: 20107143
Ther Deliv. 2017 Jul;8(7):577-595
pubmed: 28633594
Front Microbiol. 2017 Jun 13;8:1040
pubmed: 28659878
Science. 2014 Jan 10;343(6167):183-6
pubmed: 24408433
Biochim Biophys Acta. 2000 Sep 29;1468(1-2):175-86
pubmed: 11018662
Proc Natl Acad Sci U S A. 2010 Nov 2;107(44):19002-7
pubmed: 20956325
Biophys J. 2016 Oct 4;111(7):1528-1540
pubmed: 27705775
Microb Biotechnol. 2011 Mar;4(2):286-99
pubmed: 21342473
Microb Pathog. 2017 Sep;110:1-6
pubmed: 28634130
PLoS One. 2009;4(4):e5219
pubmed: 19381331
J Am Chem Soc. 2005 Jul 6;127(26):9372-3
pubmed: 15984856
Nat Commun. 2018 Apr 11;9(1):1379
pubmed: 29643357
Pediatr Obes. 2017 Jun;12(3):229-238
pubmed: 27059430
Microbiol Mol Biol Rev. 2010 Mar;74(1):81-94
pubmed: 20197500
Vaccine. 2016 Jun 14;34(28):3303-9
pubmed: 27151884
Proteomics. 2009 Dec;9(24):5425-36
pubmed: 19834908
J Bacteriol. 2014 Jul;196(13):2355-66
pubmed: 24748612
Expert Rev Proteomics. 2009 Jun;6(3):267-83
pubmed: 19489699
FEMS Microbiol Rev. 1997 Jul;20(3-4):291-303
pubmed: 9299708
J Bacteriol. 1962 Dec;84(6):1260-7
pubmed: 16561982
Gynecol Oncol. 2008 Jul;110(1):13-21
pubmed: 18589210
Nat Cell Biol. 2007 Jun;9(6):654-9
pubmed: 17486113
J Bacteriol. 1999 Aug;181(16):4725-33
pubmed: 10438737
J Appl Crystallogr. 2014 May 10;47(Pt 3):1132-1139
pubmed: 24904247
Sci Rep. 2019 Jan 29;9(1):877
pubmed: 30696852
Int J Med Microbiol. 2014 May;304(3-4):431-43
pubmed: 24631214
J Infect Dis. 2000 Mar;181(3):1034-43
pubmed: 10720528
Appl Opt. 2003 Jan 20;42(3):436-44
pubmed: 12570265
Biotechnol J. 2015 Sep;10(11):1689-706
pubmed: 26912077
PLoS One. 2013 Aug 20;8(8):e73196
pubmed: 23977379
Front Oncol. 2017 Nov 07;7:253
pubmed: 29164053
Protein Cell. 2012 Jan;3(1):28-37
pubmed: 22314808