In vivo dynamic analysis of BMP-2-induced ectopic bone formation.
Animals
Bone Morphogenetic Protein 2
/ genetics
Bone and Bones
/ diagnostic imaging
Calcification, Physiologic
/ drug effects
Cell Differentiation
/ drug effects
Collagen
/ metabolism
Mice, Transgenic
Molecular Imaging
/ methods
Neovascularization, Physiologic
/ drug effects
Osteoblasts
/ physiology
Osteoclasts
/ physiology
Osteogenesis
/ drug effects
Peptide Fragments
/ pharmacology
Teriparatide
/ analogs & derivatives
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
16 03 2020
16 03 2020
Historique:
received:
14
10
2019
accepted:
03
03
2020
entrez:
18
3
2020
pubmed:
18
3
2020
medline:
15
12
2020
Statut:
epublish
Résumé
Bone morphogenetic protein (BMP)-2 plays a central role in bone-tissue engineering because of its potent bone-induction ability. However, the process of BMP-induced bone formation in vivo remains poorly elucidated. Here, we aimed to establish a method for intravital imaging of the entire process of BMP-2-induced ectopic bone formation. Using multicolor intravital imaging in transgenic mice, we visualized the spatiotemporal process of bone induction, including appearance and motility of osteoblasts and osteoclasts, angiogenesis, collagen-fiber formation, and bone-mineral deposition. Furthermore, we investigated how PTH1-34 affects BMP-2-induced bone formation, which revealed that PTH1-34 administration accelerated differentiation and increased the motility of osteoblasts, whereas it decreased morphological changes in osteoclasts. This is the first report on visualization of the entire process of BMP-2-induced bone formation using intravital imaging techniques, which, we believe, will contribute to our understanding of ectopic bone formation and provide new parameters for evaluating bone-forming activity.
Identifiants
pubmed: 32179857
doi: 10.1038/s41598-020-61825-2
pii: 10.1038/s41598-020-61825-2
pmc: PMC7076033
doi:
Substances chimiques
Bmp2 protein, mouse
0
Bone Morphogenetic Protein 2
0
Peptide Fragments
0
Teriparatide
10T9CSU89I
Collagen
9007-34-5
parathyroid hormone (1-34)amide
GKK29YSF88
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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