The expression of cytokines in aqueous humor of high myopic patients with cataracts.
Aged
Angiopoietin-1
/ metabolism
Aqueous Humor
/ enzymology
Cataract
/ metabolism
Chemokine CXCL16
/ metabolism
Cytokines
/ metabolism
Decorin
/ metabolism
Extracellular Matrix Proteins
/ metabolism
Female
Gene Expression Regulation
/ genetics
Humans
Insulin-Like Growth Factor Binding Protein 2
/ metabolism
Interleukin-17
/ metabolism
Male
Matrix Metalloproteinase 2
/ metabolism
Middle Aged
Myopia
/ metabolism
NF-kappa B
/ metabolism
Transforming Growth Factor beta
/ metabolism
Journal
Molecular vision
ISSN: 1090-0535
Titre abrégé: Mol Vis
Pays: United States
ID NLM: 9605351
Informations de publication
Date de publication:
2020
2020
Historique:
received:
28
07
2019
accepted:
03
03
2020
entrez:
18
3
2020
pubmed:
18
3
2020
medline:
5
2
2021
Statut:
epublish
Résumé
To analyze the expression of 440 human cytokines in aqueous humor of high myopic patients with cataracts. Eighty-five patients with cataracts were recruited in this study. In the screening stage, the RayBio G-Series Human Cytokine Antibody Array 440 was used to assay the aqueous humor samples collected from nine high myopic patients with cataracts and eight non-myopic patients with cataracts right before the surgery. The array was further used for verification of the screened cytokines, with aqueous humor samples obtained from 34 eyes of high myopic patients with cataracts and 34 eyes of non-myopic patients with cataracts. Compared with the non-myopic patients with cataracts, the expression levels of decorin, receptor activator of NF-kB (RANK), angiopoietin-1 (ANG-1), C-X-C motif ligand 16 (CXCL16), β-inducible gene-h3 (bIG-H3), insulin-like growth factor-binding protein 2 (IGFBP-2), and interleukin-17B (IL-17B) were statistically significantly higher in high myopic patients with cataracts (all p<0.000114). The matrix metalloproteinase-2 (MMP-2) level also increased in the aqueous humor of high myopic patients with cataracts (p = 0.0034). The concentrations of ANG-1 and MMP-2 were also increased in the aqueous humor of the confirmatory stage (all p<0.05). In this study, numerous cytokines in aqueous humor were detected in high myopic patients with cataracts and non-myopic patients with cataracts, and it was confirmed that the MMP-2 level in the aqueous humor of patients with high myopia was statistically significantly increased. Further verification also revealed the elevation of ANG-1 in the aqueous humor of high myopic patients with cataracts, which suggests that ANG-1 may be related to the pathogenesis of high myopia.
Substances chimiques
Angiopoietin-1
0
CXCL16 protein, human
0
Chemokine CXCL16
0
Cytokines
0
Decorin
0
Extracellular Matrix Proteins
0
IGFBP2 protein, human
0
Insulin-Like Growth Factor Binding Protein 2
0
Interleukin-17
0
NF-kappa B
0
Transforming Growth Factor beta
0
betaIG-H3 protein
148710-76-3
Matrix Metalloproteinase 2
EC 3.4.24.24
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
150-157Informations de copyright
Copyright © 2020 Molecular Vision.
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