Cell function profiling to assess clone stability.
CHO
bioprocessing
cell function
clone stability
monitoring
phenotyping
process
Journal
Biotechnology and bioengineering
ISSN: 1097-0290
Titre abrégé: Biotechnol Bioeng
Pays: United States
ID NLM: 7502021
Informations de publication
Date de publication:
07 2020
07 2020
Historique:
received:
24
09
2019
revised:
24
01
2020
accepted:
16
03
2020
pubmed:
18
3
2020
medline:
17
8
2021
entrez:
18
3
2020
Statut:
ppublish
Résumé
In cell line development the identification of stable Chinese hamster ovary cells for production is a critical but onerous task. The stability trial focus upon high-level attributes can mask profound underlying cellular changes, leading to unstable clones mistakenly being chosen for production. The challenge is to assay underlying cell pathways and subsystems without pushing up cell line development costs. ChemStress® cell function profiling is a simple, multiwell plate-based assay that uses a panel of active chemicals to mimic known bioprocess stresses and challenge key pathways. After 3 days of static culture on the plate, functional responses are assayed, for example, titer and growth. Here this approach is used to monitor 131 clones as they change over real stability trials. A novel stability metric is defined over the data to identify stable clones that remain unperturbed across many components of cell function. This allows stability trials to look beneath the titer to identify clones that are internally more stable.
Identifiants
pubmed: 32181887
doi: 10.1002/bit.27336
pmc: PMC7383652
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2295-2299Subventions
Organisme : H2020 Innovation In SMEs
ID : 778586
Pays : International
Informations de copyright
© 2020 The Authors. Biotechnology and Bioengineering published by Wiley Periodicals, Inc.
Références
ACS Synth Biol. 2018 Sep 21;7(9):2148-2159
pubmed: 30060646
Biotechnol Bioeng. 2010 Aug 15;106(6):938-51
pubmed: 20589672
Biotechnol Bioeng. 2003 Mar 20;81(6):631-9
pubmed: 12529877
Biotechnol J. 2018 Mar;13(3):e1700230
pubmed: 28865132
Bioinformatics. 2003 Oct 12;19(15):1934-6
pubmed: 14555626
Biotechnol Bioeng. 2020 Jul;117(7):2295-2299
pubmed: 32181887
Anal Biochem. 2017 Oct 1;534:49-55
pubmed: 28716374
J Proteome Res. 2014 Jul 3;13(7):3144-59
pubmed: 24915626
Biotechnol Bioeng. 2013 Jan;110(1):260-74
pubmed: 22833427
Biotechnol Bioeng. 2018 Mar;115(3):645-660
pubmed: 29080362