A multi-layered structure of the interphase chromocenter revealed by proximity-based biotinylation.
Adenosine Triphosphatases
/ metabolism
Animals
Biotinylation
Cell Cycle Proteins
/ analysis
Cell Line
Cell Nucleus
/ metabolism
Centromere
/ metabolism
Centromere Protein A
/ genetics
Chromatin
/ metabolism
Chromatin Immunoprecipitation Sequencing
Chromobox Protein Homolog 5
Chromosomal Proteins, Non-Histone
/ analysis
DNA-Binding Proteins
/ metabolism
Drosophila
/ genetics
Drosophila Proteins
/ genetics
Interphase
/ genetics
Multiprotein Complexes
/ metabolism
Proteomics
Recombinant Fusion Proteins
/ analysis
Cohesins
Journal
Nucleic acids research
ISSN: 1362-4962
Titre abrégé: Nucleic Acids Res
Pays: England
ID NLM: 0411011
Informations de publication
Date de publication:
07 05 2020
07 05 2020
Historique:
accepted:
25
02
2020
revised:
18
02
2020
received:
17
10
2019
pubmed:
18
3
2020
medline:
28
7
2020
entrez:
18
3
2020
Statut:
ppublish
Résumé
During interphase centromeres often coalesce into a small number of chromocenters, which can be visualized as distinct, DAPI dense nuclear domains. Intact chromocenters play a major role in maintaining genome stability as they stabilize the transcriptionally silent state of repetitive DNA while ensuring centromere function. Despite its biological importance, relatively little is known about the molecular composition of the chromocenter or the processes that mediate chromocenter formation and maintenance. To provide a deeper molecular insight into the composition of the chromocenter and to demonstrate the usefulness of proximity-based biotinylation as a tool to investigate those questions, we performed super resolution microscopy and proximity-based biotinylation experiments of three distinct proteins associated with the chromocenter in Drosophila. Our work revealed an intricate internal architecture of the chromocenter suggesting a complex multilayered structure of this intranuclear domain.
Identifiants
pubmed: 32182352
pii: 5809156
doi: 10.1093/nar/gkaa145
pmc: PMC7192626
doi:
Substances chimiques
Cell Cycle Proteins
0
Centromere Protein A
0
Chromatin
0
Chromosomal Proteins, Non-Histone
0
Cid protein, Drosophila
0
DNA-Binding Proteins
0
Drosophila Proteins
0
Hmr protein, Drosophila
0
Multiprotein Complexes
0
Recombinant Fusion Proteins
0
condensin complexes
0
Chromobox Protein Homolog 5
107283-02-3
Adenosine Triphosphatases
EC 3.6.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4161-4178Informations de copyright
© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.
Références
Elife. 2016 Aug 11;5:
pubmed: 27514026
Mol Cell. 2014 Jul 17;55(2):332-41
pubmed: 25002142
Nature. 2007 Nov 8;450(7167):203-18
pubmed: 17994087
Proc Natl Acad Sci U S A. 2003 Apr 29;100(9):5302-7
pubmed: 12695567
Annu Rev Cell Dev Biol. 2013;29:241-70
pubmed: 23834025
Science. 2013 Mar 15;339(6125):1328-1331
pubmed: 23371551
Mol Cell Biol. 2004 Jul;24(14):6393-402
pubmed: 15226439
PLoS Genet. 2020 Mar 23;16(3):e1008673
pubmed: 32203508
Science. 2009 Oct 9;326(5950):289-93
pubmed: 19815776
Nature. 1975 Aug 7;256(5517):495-7
pubmed: 1172191
Nat Cell Biol. 2006 May;8(5):427-9
pubmed: 16691204
Genome Res. 2012 Nov;22(11):2176-87
pubmed: 22722341
Nucleic Acids Res. 2016 Mar 18;44(5):2145-59
pubmed: 26586808
Epigenetics Chromatin. 2016 Dec 24;9:57
pubmed: 28035241
PLoS Genet. 2018 Jul 12;14(7):e1007393
pubmed: 30001329
Nature. 1970 Mar 7;225(5236):912-5
pubmed: 5415126
Proteomics. 2014 Oct;14(19):2167-78
pubmed: 24841622
PLoS Biol. 2012;10(11):e1001428
pubmed: 23189033
Methods Mol Biol. 2000;132:365-86
pubmed: 10547847
J Cell Sci. 2005 Jun 1;118(Pt 11):2529-43
pubmed: 15923665
Nature. 2017 Jul 13;547(7662):241-245
pubmed: 28636597
Nat Biotechnol. 2014 Mar;32(3):223-6
pubmed: 24727771
Chromosoma. 1970;31(3):331-45
pubmed: 5489364
Mol Biol Cell. 2018 May 15;29(10):1258-1269
pubmed: 29540532
PLoS Biol. 2014 Oct 07;12(10):e1001962
pubmed: 25290697
Nucleic Acids Res. 2012 Dec;40(22):11178-88
pubmed: 23066104
Cell Rep. 2016 May 31;15(9):2038-49
pubmed: 27210764
Development. 2018 Dec 4;145(23):
pubmed: 30389853
Nature. 2017 Jul 13;547(7662):236-240
pubmed: 28636604
Nat Rev Mol Cell Biol. 2007 Apr;8(4):284-95
pubmed: 17380162
Science. 2003 Jan 31;299(5607):719-21
pubmed: 12560554
Trends Genet. 2018 Aug;34(8):587-599
pubmed: 29871772
Nucleic Acids Res. 2018 Nov 30;46(21):11274-11286
pubmed: 30357352
Genes Dev. 2014 Jul 1;28(13):1445-60
pubmed: 24990964
Elife. 2018 Mar 26;7:
pubmed: 29578410
Mol Cell. 2018 Feb 1;69(3):517-532.e11
pubmed: 29395067
Cell. 2018 May 3;173(4):972-988.e23
pubmed: 29656893
Data Brief. 2015 Jul 26;4:544-50
pubmed: 26306323
Cell. 2011 Oct 28;147(3):690-703
pubmed: 22036573
Mol Biol Evol. 2019 Aug 1;36(8):1783-1792
pubmed: 31038678
Elife. 2019 Feb 11;8:
pubmed: 30741633
J Mol Biol. 2015 Feb 13;427(3):608-25
pubmed: 25280896
Chromosoma. 2017 Dec;126(6):697-712
pubmed: 28688038
Biochemistry (Mosc). 2002 Apr;67(4):423-31
pubmed: 11996655
Genetics. 2017 Dec;207(4):1457-1472
pubmed: 29046402
Cell. 2018 Jan 25;172(3):590-604.e13
pubmed: 29373831
Biophys J. 2009 Dec 2;97(11):2876-85
pubmed: 19948116
G3 (Bethesda). 2019 Feb 7;9(2):345-357
pubmed: 30514714
Science. 2013 Jun 7;340(6137):1211-4
pubmed: 23744945
J Cell Biol. 2015 Dec 21;211(6):1141-56
pubmed: 26668330
Cell. 1998 Feb 20;92(4):511-21
pubmed: 9491892
Cell. 2009 Sep 18;138(6):1067-82
pubmed: 19766562
Genetics. 2009 Apr;181(4):1437-50
pubmed: 19153254
Nature. 2002 May 16;417(6886):227
pubmed: 12015578
Genome Biol. 2014 Jun 30;15(6):R82
pubmed: 24981874
Trends Ecol Evol. 2013 Jan;28(1):48-57
pubmed: 22995895
EMBO J. 1997 Sep 1;16(17):5280-8
pubmed: 9311988
Mol Cell. 2000 Nov;6(5):1025-35
pubmed: 11106742
J Cell Sci. 2004 Aug 1;117(Pt 17):3707-11
pubmed: 15286171
PLoS Genet. 2012;8(3):e1002597
pubmed: 22457639
Dev Cell. 2007 Jan;12(1):45-55
pubmed: 17199040
Science. 2009 Dec 11;326(5959):1538-41
pubmed: 19933102
Cell Rep. 2019 Mar 12;26(11):2890-2903.e3
pubmed: 30865881
Cell. 2001 Nov 2;107(3):323-37
pubmed: 11701123
G3 (Bethesda). 2017 Feb 9;7(2):693-704
pubmed: 28007840
J Cell Biol. 2014 Nov 10;207(3):335-49
pubmed: 25365994
Life Sci Alliance. 2018 Sep 17;1(5):e201800179
pubmed: 30456388
Mol Cell. 2013 Apr 25;50(2):236-49
pubmed: 23562326
Nucleic Acids Res. 2014 Jun;42(11):e89
pubmed: 24748663
Nat Cell Biol. 2006 May;8(5):458-69
pubmed: 16622419
Dev Cell. 2013 Nov 25;27(4):412-24
pubmed: 24239514
Mol Plant. 2016 Aug 1;9(8):1142-1155
pubmed: 27150037
PLoS Genet. 2014 Mar 20;10(3):e1004240
pubmed: 24651406
Mol Cell Biol. 2004 Apr;24(8):3157-67
pubmed: 15060140
Genetics. 1997 Apr;145(4):1031-40
pubmed: 9093855
Genetics. 2018 Mar;208(3):977-990
pubmed: 29305387
Dev Cell. 2010 Oct 19;19(4):625-38
pubmed: 20951352
Nat Methods. 2018 Jun;15(6):433-436
pubmed: 29735996
Mol Cell. 2020 Jan 2;77(1):67-81.e7
pubmed: 31784359
Proc Natl Acad Sci U S A. 2002 Oct 29;99(22):14584-9
pubmed: 12384572
PLoS One. 2017 Feb 16;12(2):e0171798
pubmed: 28207793
Nat Methods. 2015 Jan;12(1):51-4
pubmed: 25419960
Dev Cell. 2006 Mar;10(3):303-15
pubmed: 16516834
Mol Cell. 2008 Oct 10;32(1):1-9
pubmed: 18851828
Nucleic Acids Res. 2017 Jan 4;45(D1):D362-D368
pubmed: 27924014
Int J Evol Biol. 2012;2012:301894
pubmed: 22191063
BMC Genomics. 2018 Feb 20;19(1):151
pubmed: 29458329
J Cell Biol. 2018 Jun 4;217(6):1957-1972
pubmed: 29626011
Mol Cell Biol. 1986 Nov;6(11):3862-72
pubmed: 3099166
Dev Cell. 2015 Nov 23;35(4):497-512
pubmed: 26585297
Chromosoma. 2011 Feb;120(1):83-96
pubmed: 20862486
PLoS Comput Biol. 2014 Oct 23;10(10):e1003867
pubmed: 25340767
Science. 2003 Jan 31;299(5607):721-5
pubmed: 12560555
Dev Cell. 2015 Jul 6;34(1):73-84
pubmed: 26151904
J Cell Biol. 2008 Dec 1;183(5):805-18
pubmed: 19047461
Genetics. 2007 Dec;177(4):2233-41
pubmed: 17947412
Cell Rep. 2013 Sep 26;4(6):1156-67
pubmed: 24055057
Genes Dev. 2005 Sep 1;19(17):2041-53
pubmed: 16140985
Genet Res Int. 2012;2012:430136
pubmed: 22567387