Oxygen Tension and the VHL-Hif1α Pathway Determine Onset of Neuronal Polarization and Cerebellar Germinal Zone Exit.
Animals
Cell Differentiation
/ physiology
Cell Polarity
/ physiology
Cerebellum
/ growth & development
Female
Hypoxia-Inducible Factor 1, alpha Subunit
/ metabolism
Male
Mice
Neurogenesis
/ physiology
Neurons
/ cytology
Oxygen
Signal Transduction
/ physiology
Von Hippel-Lindau Tumor Suppressor Protein
/ metabolism
Hif1 Pathway
Integrin
Pard Complex
Zeb1
cell polarity
neuronal differentiation
vascularization
Journal
Neuron
ISSN: 1097-4199
Titre abrégé: Neuron
Pays: United States
ID NLM: 8809320
Informations de publication
Date de publication:
20 05 2020
20 05 2020
Historique:
received:
12
12
2019
revised:
04
02
2020
accepted:
19
02
2020
pubmed:
19
3
2020
medline:
22
8
2020
entrez:
19
3
2020
Statut:
ppublish
Résumé
Postnatal brain circuit assembly is driven by temporally regulated intrinsic and cell-extrinsic cues that organize neurogenesis, migration, and axo-dendritic specification in post-mitotic neurons. While cell polarity is an intrinsic organizer of morphogenic events, environmental cues in the germinal zone (GZ) instructing neuron polarization and their coupling during postnatal development are unclear. We report that oxygen tension, which rises at birth, and the von Hippel-Lindau (VHL)-hypoxia-inducible factor 1α (Hif1α) pathway regulate polarization and maturation of post-mitotic cerebellar granule neurons (CGNs). At early postnatal stages with low GZ vascularization, Hif1α restrains CGN-progenitor cell-cycle exit. Unexpectedly, cell-intrinsic VHL-Hif1α pathway activation also delays the timing of CGN differentiation, germinal zone exit, and migration initiation through transcriptional repression of the partitioning-defective (Pard) complex. As vascularization proceeds, these inhibitory mechanisms are downregulated, implicating increasing oxygen tension as a critical switch for neuronal polarization and cerebellar GZ exit.
Identifiants
pubmed: 32183943
pii: S0896-6273(20)30148-3
doi: 10.1016/j.neuron.2020.02.025
pmc: PMC7244394
mid: NIHMS1574245
pii:
doi:
Substances chimiques
Hypoxia-Inducible Factor 1, alpha Subunit
0
Von Hippel-Lindau Tumor Suppressor Protein
EC 2.3.2.27
Oxygen
S88TT14065
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
607-623.e5Subventions
Organisme : Medical Research Council
ID : MC_PC_17230
Pays : United Kingdom
Organisme : NINDS NIH HHS
ID : P01 NS083513
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS104029
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS066936
Pays : United States
Organisme : Medical Research Council
ID : MC_PC_12009
Pays : United Kingdom
Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests.
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