Analyzing electron transport chain supercomplexes.

Cytological Techniques / methods Electron Transport Electron Transport Chain Complex Proteins / metabolism Electrophoresis, Polyacrylamide Gel Oxygen Consumption
Blue native gel electrophoresis Electron transport chain Mitochondria respiration Oxidative phosphorylation Supercomplexes

Journal

Methods in cell biology
ISSN: 0091-679X
Titre abrégé: Methods Cell Biol
Pays: United States
ID NLM: 0373334

Informations de publication

Date de publication:
2020
Historique:
entrez: 19 3 2020
pubmed: 19 3 2020
medline: 29 12 2020
Statut: ppublish

Résumé

This review focuses on three independent and complementary approaches to obtain information on the combined function of respiratory complexes when present in different structural situations, either as individual complexes or when superassembled with other complexes. We review the utility of in-gel activity after blue native electrophoresis, integrated oxygen consumption of supercomplexes containing complex IV, and spectrophotometric activity measurements.

Identifiants

DOI: 10.1016/bs.mcb.2019.12.002 PMID: 32183958
pubmed: 32183958
pii: S0091-679X(19)30157-8
doi: 10.1016/bs.mcb.2019.12.002
pii:
doi:

Substances chimiques

Electron Transport Chain Complex Proteins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

181-197

Informations de copyright

© 2020 Elsevier Inc. All rights reserved.

Auteurs

Rebeca Acín-Pérez (R)

Fundación Centro Nacional de Investigaciones Cardiovasculares Carlos III, Madrid, Spain.

Pablo Hernansanz-Agustín (P)

Fundación Centro Nacional de Investigaciones Cardiovasculares Carlos III, Madrid, Spain.

José Antonio Enríquez (JA)

Fundación Centro Nacional de Investigaciones Cardiovasculares Carlos III, Madrid, Spain. Electronic address: jaenriquez@cnic.es.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques cytologiques › Analyzing electron transport chain supercomplexes.
questionsmedicales.fr › Métabolisme › Voies et réseaux métaboliques › Transport d'électrons › Analyzing electron transport chain supercomplexes.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Complexe enzymatique de la chaine respiratoire mitochondriale › Analyzing electron transport chain supercomplexes.
questionsmedicales.fr › Techniques d'investigation › Techniques électrochimiques › Électrophorèse › Électrophorèse sur gel de polyacrylamide › Analyzing electron transport chain supercomplexes.
questionsmedicales.fr › Métabolisme › Consommation d'oxygène › Analyzing electron transport chain supercomplexes.