Glucose availability but not changes in pancreatic hormones sensitizes hepatic AMPK activity during nutritional transition in rodents.
AMP-activated kinase (AMPK)
energy homeostasis
glucagon
glucose
hepatocyte
insulin
liver
metabolic regulation
metformin
nutrient sensing
nutritional transition
Journal
The Journal of biological chemistry
ISSN: 1083-351X
Titre abrégé: J Biol Chem
Pays: United States
ID NLM: 2985121R
Informations de publication
Date de publication:
01 05 2020
01 05 2020
Historique:
received:
17
07
2019
revised:
10
03
2020
pubmed:
19
3
2020
medline:
29
12
2020
entrez:
19
3
2020
Statut:
ppublish
Résumé
The cellular energy sensor AMP-activated protein kinase (AMPK) is a metabolic regulator that mediates adaptation to nutritional variations to maintain a proper energy balance in cells. We show here that suckling-weaning and fasting-refeeding transitions in rodents are associated with changes in AMPK activation and the cellular energy state in the liver. These nutritional transitions were characterized by a metabolic switch from lipid to glucose utilization, orchestrated by modifications in glucose levels and the glucagon/insulin ratio in the bloodstream. We therefore investigated the respective roles of glucose and pancreatic hormones on AMPK activation in mouse primary hepatocytes. We found that glucose starvation transiently activates AMPK, whereas changes in glucagon and insulin levels had no impact on AMPK. Challenge of hepatocytes with metformin-induced metabolic stress strengthened both AMPK activation and cellular energy depletion under limited-glucose conditions, whereas neither glucagon nor insulin altered AMPK activation. Although both insulin and glucagon induced AMPKα phosphorylation at its Ser
Identifiants
pubmed: 32184359
pii: S0021-9258(17)48219-6
doi: 10.1074/jbc.RA119.010244
pmc: PMC7196663
pii:
doi:
Substances chimiques
Pancreatic Hormones
0
AMP-Activated Protein Kinases
EC 2.7.11.31
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
5836-5849Informations de copyright
© 2020 Huet et al.
Références
J Clin Invest. 2010 Jul;120(7):2355-69
pubmed: 20577053
J Comp Pathol. 2011 Jul;145(1):88-94
pubmed: 21255792
Proc Natl Acad Sci U S A. 2010 Jun 22;107(25):11549-54
pubmed: 20534540
Am J Physiol Endocrinol Metab. 2005 Nov;289(5):E794-800
pubmed: 15956049
J Biol Chem. 1967 Jul 10;242(13):3239-41
pubmed: 6027798
Biochem Biophys Res Commun. 2011 Jan 7;404(1):382-7
pubmed: 21130749
Nat Med. 2013 Dec;19(12):1649-54
pubmed: 24185692
J Biol Chem. 2006 Mar 3;281(9):5335-40
pubmed: 16340011
Science. 2016 Jan 15;351(6270):275-281
pubmed: 26816379
Diabetes. 2006 Mar;55(3):849-55
pubmed: 16505254
J Biol Chem. 2003 Aug 1;278(31):28434-42
pubmed: 12764152
J Biol Chem. 2006 Dec 1;281(48):36662-72
pubmed: 17023420
J Biol Chem. 2014 Feb 28;289(9):5950-9
pubmed: 24403081
EBioMedicine. 2018 Feb;28:194-209
pubmed: 29343420
Cell Metab. 2006 Jun;3(6):403-16
pubmed: 16753576
Am J Physiol Endocrinol Metab. 2006 Mar;290(3):E471-9
pubmed: 16234268
J Clin Invest. 2004 Jul;114(2):232-9
pubmed: 15254590
Biochim Biophys Acta. 1982 Dec 13;713(3):663-9
pubmed: 6129898
Cell Metab. 2014 Dec 2;20(6):939-52
pubmed: 25448702
Nat Commun. 2015 Aug 14;6:7926
pubmed: 26272043
Biochem J. 2014 Apr 15;459(2):275-87
pubmed: 24467442
PLoS One. 2017 Jan 20;12(1):e0170382
pubmed: 28107516
PLoS One. 2012;7(1):e29887
pubmed: 22253816
J Biol Chem. 1992 Feb 15;267(5):2864-7
pubmed: 1346611
Diabetes. 2006 Apr;55(4):919-23
pubmed: 16567511
Biochem J. 1991 Dec 15;280 ( Pt 3):733-7
pubmed: 1684893
Cell Metab. 2008 Nov;8(5):359-71
pubmed: 19046568
Cell Metab. 2013 Oct 1;18(4):546-55
pubmed: 24093678
EBioMedicine. 2018 May;31:122-132
pubmed: 29673898
Biochem J. 2017 Aug 22;474(17):3059-3073
pubmed: 28694351
Chem Biol. 2014 May 22;21(5):619-27
pubmed: 24746562
J Biol Chem. 2004 Dec 24;279(52):54103-9
pubmed: 15494402
Crit Rev Biochem Mol Biol. 2010 Aug;45(4):276-95
pubmed: 20522000
Biochem Biophys Res Commun. 2005 Jan 28;326(4):851-8
pubmed: 15607747
Biochem J. 2001 Mar 1;354(Pt 2):275-83
pubmed: 11171104
Nature. 2017 Aug 3;548(7665):112-116
pubmed: 28723898
Hepatol Commun. 2018 Nov 12;3(1):84-98
pubmed: 30619997
Int J Mol Sci. 2018 Sep 19;19(9):
pubmed: 30235785
Mol Genet Metab. 2008 Feb;93(2):200-9
pubmed: 17950019
Diabetologia. 2004 Nov;47(11):2012-21
pubmed: 15578153
Arch Biochem Biophys. 2017 Jun 1;623-624:49-57
pubmed: 28526426
Diabetes. 2005 May;54(5):1331-9
pubmed: 15855317
Mol Cell Endocrinol. 2013 Feb 25;366(2):152-62
pubmed: 22796337
Biochem J. 2000 Jun 15;348 Pt 3:607-14
pubmed: 10839993
Am J Physiol Endocrinol Metab. 2016 Oct 1;311(4):E730-E740
pubmed: 27577854
Biochem J. 2007 Jun 15;404(3):499-507
pubmed: 17324122
Cell Res. 2019 Jun;29(6):460-473
pubmed: 30948787
Cell Signal. 2015 May;27(5):978-88
pubmed: 25683918
J Clin Invest. 2013 Jul;123(7):2764-72
pubmed: 23863634
Methods Mol Biol. 2018;1732:229-237
pubmed: 29480479
J Clin Invest. 2009 Aug;119(8):2412-22
pubmed: 19662685
Proc Natl Acad Sci U S A. 2010 Nov 9;107(45):19237-41
pubmed: 20974912
J Biol Chem. 2000 Jan 7;275(1):223-8
pubmed: 10617608