Simultaneous Determination of Isoniazid, Pyrazinamide and Rifampin in Human Plasma by High-performance Liquid Chromatography and UV Detection.
Anti-TB drugs
HPLC-UV
Isoniazid
Pyrazinamide
Rifampin
Therapeutic drug monitoring
Journal
Iranian journal of pharmaceutical research : IJPR
ISSN: 1735-0328
Titre abrégé: Iran J Pharm Res
Pays: Netherlands
ID NLM: 101208407
Informations de publication
Date de publication:
2019
2019
Historique:
entrez:
19
3
2020
pubmed:
19
3
2020
medline:
19
3
2020
Statut:
ppublish
Résumé
Therapeutic Drug Monitoring (TDM) of first-line anti-tuberculosis (TB) drugs is a decisive tool, allowing the clinician to successfully treat TB patients. The objective of the study was to develop and optimize a simple, sensitive, and reliable high-performance liquid chromatography (HPLC) method for the simultaneous determination of isoniazid (INH), pyrazinamide (PZA), and rifampin (RIF) levels in human plasma. Nicotinamide was used as the internal standard and the samples were prepared after protein precipitation using acetonitrile and zinc sulfate. The separation was achieved using a C18 reversed-phase applying gradient elution. The mobile phase was a combination of water-methanol solution with a ratio of 95:05 (v/v) at the initial phase. All calibration curves had good linearity (r
Identifiants
pubmed: 32184842
doi: 10.22037/ijpr.2019.1100849
pmc: PMC7059034
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1735-1741Références
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