Cancer Cells Resist Mechanical Destruction in Circulation via RhoA/Actomyosin-Dependent Mechano-Adaptation.
Actomyosin
/ metabolism
Adaptation, Biological
Animals
Cell Line, Tumor
Cell Membrane
/ metabolism
Cell Survival
Hemodynamics
Humans
Mice, Inbred C57BL
Myosin Type II
/ metabolism
Neoplasm Metastasis
Neoplasms
/ metabolism
Neoplastic Cells, Circulating
/ pathology
Shear Strength
Stress, Mechanical
rhoA GTP-Binding Protein
/ metabolism
RhoA
circulating tumor cells
fluid shear stress
formin
metastasis
myosin II
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
17 03 2020
17 03 2020
Historique:
received:
03
10
2019
revised:
31
01
2020
accepted:
20
02
2020
entrez:
19
3
2020
pubmed:
19
3
2020
medline:
24
3
2021
Statut:
ppublish
Résumé
During metastasis, cancer cells are exposed to potentially destructive hemodynamic forces including fluid shear stress (FSS) while en route to distant sites. However, prior work indicates that cancer cells are more resistant to brief pulses of high-level FSS in vitro relative to non-transformed epithelial cells. Herein, we identify a mechano-adaptive mechanism of FSS resistance in cancer cells. Our findings demonstrate that cancer cells activate RhoA in response to FSS, which protects them from FSS-induced plasma membrane damage. We show that cancer cells freshly isolated from mouse and human tumors are resistant to FSS, that formin and myosin II activity protects circulating tumor cells (CTCs) from destruction, and that short-term inhibition of myosin II delays metastasis in mouse models. Collectively, our data indicate that viable CTCs actively resist destruction by hemodynamic forces and are likely to be more mechanically robust than is commonly thought.
Identifiants
pubmed: 32187555
pii: S2211-1247(20)30254-0
doi: 10.1016/j.celrep.2020.02.080
pmc: PMC7219793
mid: NIHMS1578463
pii:
doi:
Substances chimiques
RHOA protein, human
124671-05-2
Actomyosin
9013-26-7
Myosin Type II
EC 3.6.1.-
rhoA GTP-Binding Protein
EC 3.6.5.2
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
3864-3874.e6Subventions
Organisme : NCI NIH HHS
ID : R21 CA179981
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM067795
Pays : United States
Organisme : NCI NIH HHS
ID : R21 CA196202
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA086862
Pays : United States
Organisme : NCI NIH HHS
ID : P50 CA174521
Pays : United States
Informations de copyright
Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests M.D.H. is president, co-founder, and shareholder of SynderBio, Inc.
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