Defining Epidermal Basal Cell States during Skin Homeostasis and Wound Healing Using Single-Cell Transcriptomics.
FLIM
basal cell state
cellular transition dynamics
epidermis
metabolism
plasticity
single-cell RNA sequencing
skin
stem cell
wound healing
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
17 03 2020
17 03 2020
Historique:
received:
14
09
2019
revised:
07
01
2020
accepted:
25
02
2020
entrez:
19
3
2020
pubmed:
19
3
2020
medline:
24
3
2021
Statut:
ppublish
Résumé
Our knowledge of transcriptional heterogeneities in epithelial stem and progenitor cell compartments is limited. Epidermal basal cells sustain cutaneous tissue maintenance and drive wound healing. Previous studies have probed basal cell heterogeneity in stem and progenitor potential, but a comprehensive dissection of basal cell dynamics during differentiation is lacking. Using single-cell RNA sequencing coupled with RNAScope and fluorescence lifetime imaging, we identify three non-proliferative and one proliferative basal cell state in homeostatic skin that differ in metabolic preference and become spatially partitioned during wound re-epithelialization. Pseudotemporal trajectory and RNA velocity analyses predict a quasi-linear differentiation hierarchy where basal cells progress from Col17a1
Identifiants
pubmed: 32187560
pii: S2211-1247(20)30265-5
doi: 10.1016/j.celrep.2020.02.091
pmc: PMC7218802
mid: NIHMS1578466
pii:
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
3932-3947.e6Subventions
Organisme : NIAMS NIH HHS
ID : R01 AR068074
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM123731
Pays : United States
Organisme : NIGMS NIH HHS
ID : P41 GM103540
Pays : United States
Organisme : NIAMS NIH HHS
ID : U01 AR073159
Pays : United States
Organisme : NIAMS NIH HHS
ID : P30 AR075047
Pays : United States
Informations de copyright
Copyright © 2020 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests.
Références
J Invest Dermatol. 2012 Jun;132(6):1543-53
pubmed: 22377760
Biostatistics. 2007 Jan;8(1):118-27
pubmed: 16632515
Biophys J. 2008 Jan 15;94(2):L14-6
pubmed: 17981902
Nature. 1999 Apr 22;398(6729):714-8
pubmed: 10227294
Cell Res. 2018 Dec;28(12):1141-1157
pubmed: 30315278
Dev Cell. 2018 Oct 8;47(1):21-37.e5
pubmed: 30220568
J Cell Sci. 2005 Jun 1;118(Pt 11):2363-70
pubmed: 15923649
Nat Commun. 2017 Mar 01;8:14684
pubmed: 28248284
Cell Rep. 2015 Jan 6;10(1):1-7
pubmed: 25543138
J Invest Dermatol. 2010 Feb;130(2):388-97
pubmed: 19675579
Nature. 2018 Aug;560(7719):494-498
pubmed: 30089906
J Cell Biol. 2006 Apr 24;173(2):253-64
pubmed: 16636146
Dev Dyn. 2018 Mar;247(3):473-480
pubmed: 28795450
Commun Biol. 2018 Nov 19;1:198
pubmed: 30480099
Cell Rep. 2018 Nov 27;25(9):2457-2469.e8
pubmed: 30485812
Genes Dev. 2006 Nov 15;20(22):3185-97
pubmed: 17114587
Cell. 2017 Apr 20;169(3):483-496.e13
pubmed: 28413068
Lancet. 2001 Sep 29;358(9287):1067-8
pubmed: 11589942
Nat Genet. 2006 Nov;38(11):1335-40
pubmed: 17041601
Cell Stem Cell. 2019 Feb 7;24(2):271-284.e8
pubmed: 30686763
J Cell Sci. 2009 Sep 15;122(Pt 18):3209-13
pubmed: 19726630
Nature. 2008 May 15;453(7193):314-21
pubmed: 18480812
Cell Rep. 2018 Oct 23;25(4):871-883
pubmed: 30355494
Nature. 1999 Apr 22;398(6729):708-13
pubmed: 10227293
Cell Rep. 2018 Oct 16;25(3):585-597.e7
pubmed: 30332640
Oncogene. 2006 Mar 30;25(14):2070-81
pubmed: 16288215
Nat Methods. 2017 Oct;14(10):979-982
pubmed: 28825705
Proc Natl Acad Sci U S A. 1999 Aug 3;96(16):9089-94
pubmed: 10430900
Proc Natl Acad Sci U S A. 2007 Feb 27;104(9):3255-60
pubmed: 17360634
J Cell Commun Signal. 2016 Jun;10(2):103-20
pubmed: 27170326
Development. 2004 May;131(10):2257-68
pubmed: 15102710
Science. 2006 Jun 30;312(5782):1946-9
pubmed: 16809539
J Invest Dermatol. 2006 Apr;126(4):902-11
pubmed: 16439969
Mol Biol Cell. 2001 Nov;12(11):3439-50
pubmed: 11694579
Cell Syst. 2016 Sep 28;3(3):221-237.e9
pubmed: 27641957
Dev Dyn. 2002 Mar;223(3):371-8
pubmed: 11891986
Sci Rep. 2012;2:568
pubmed: 22891156
Nat Commun. 2017 Jun 01;8:15599
pubmed: 28569836
Nat Biotechnol. 2018 Jun;36(5):411-420
pubmed: 29608179
Biol Reprod. 2016 Dec;95(6):129
pubmed: 27683265
Genes Dev. 2013 Sep 15;27(18):1959-73
pubmed: 24029918
Microsc Res Tech. 2008 Mar;71(3):201-13
pubmed: 18008362
Science. 2014 Feb 14;343(6172):776-9
pubmed: 24531970
Annu Rev Biochem. 1991;60:281-319
pubmed: 1883198
Nat Methods. 2017 Sep 29;14(10):935-936
pubmed: 28960196
IEEE/ACM Trans Comput Biol Bioinform. 2012;9(2):487-98
pubmed: 21464514
Nat Biotechnol. 2014 Apr;32(4):381-386
pubmed: 24658644
Elife. 2017 Jul 11;6:
pubmed: 28693719
PLoS One. 2013 Oct 16;8(10):e77806
pubmed: 24147081
J Clin Invest. 2016 Nov 1;126(11):4088-4102
pubmed: 27669461
Cell. 2018 Feb 22;172(5):1091-1107.e17
pubmed: 29474909
Nature. 2019 Feb;566(7745):496-502
pubmed: 30787437
Science. 2016 Jun 17;352(6292):1471-4
pubmed: 27229141
Cell Stem Cell. 2011 Sep 2;9(3):219-32
pubmed: 21885018
Nature. 2019 Apr;568(7752):344-350
pubmed: 30944469
Nat Cell Biol. 2017 Mar 01;19(2):155-163
pubmed: 28248302
Nat Genet. 2018 Dec;50(12):1658-1665
pubmed: 30397335
J Invest Dermatol. 2008 Jul;128(7):1821-9
pubmed: 18185532
Trends Cell Biol. 2018 Sep;28(9):709-722
pubmed: 29807713
Mol Cell. 2018 Oct 4;72(1):7-9
pubmed: 30290149
Nucleic Acids Res. 2015 Jan;43(Database issue):D76-81
pubmed: 25262351
Bioinformatics. 2018 Jun 15;34(12):2077-2086
pubmed: 29415263
JCI Insight. 2017 Jun 2;2(11):
pubmed: 28570274
Nature. 2012 Sep 13;489(7415):257-62
pubmed: 22940863
Nat Rev Mol Cell Biol. 2013 Jun;14(6):329-40
pubmed: 23698583
Sci Rep. 2018 Nov 20;8(1):17094
pubmed: 30459440
J Cell Biol. 2008 Jan 28;180(2):273-84
pubmed: 18209104
Dev Cell. 2017 Nov 20;43(4):387-401
pubmed: 29161590
Int J Biochem Cell Biol. 2006;38(7):1043-9
pubmed: 16423552
Nat Cell Biol. 2016 Jun;18(6):619-31
pubmed: 27183471
Nat Biotechnol. 2015 May;33(5):495-502
pubmed: 25867923