Recent Advances in Practical Methods for Liver Cell Biology: A Short Overview.
Animals
Carcinoma, Hepatocellular
/ pathology
Cell Biology
Cell Communication
Cell Culture Techniques
Cytological Techniques
/ methods
Disease Models, Animal
Fatty Liver
/ pathology
Gene Expression
Germany
Hepatocytes
/ pathology
Humans
Hypertension, Portal
/ pathology
Liver
/ pathology
Liver Neoplasms
/ pathology
Non-alcoholic Fatty Liver Disease
/ pathology
Organoids
/ pathology
fibrogenesis
hepatic stellate cells
hepatocellular cancer
in vitro models
steatosis
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
16 Mar 2020
16 Mar 2020
Historique:
received:
06
02
2020
revised:
06
03
2020
accepted:
09
03
2020
entrez:
20
3
2020
pubmed:
20
3
2020
medline:
18
12
2020
Statut:
epublish
Résumé
Molecular and cellular research modalities for the study of liver pathologies have been tremendously improved over the recent decades. Advanced technologies offer novel opportunities to establish cell isolation techniques with excellent purity, paving the path for 2D and 3D microscopy and high-throughput assays (e.g., bulk or single-cell RNA sequencing). The use of stem cell and organoid research will help to decipher the pathophysiology of liver diseases and the interaction between various parenchymal and non-parenchymal liver cells. Furthermore, sophisticated animal models of liver disease allow for the in vivo assessment of fibrogenesis, portal hypertension and hepatocellular carcinoma (HCC) and for the preclinical testing of therapeutic strategies. The purpose of this review is to portray in detail novel in vitro and in vivo methods for the study of liver cell biology that had been presented at the workshop of the 8th meeting of the European Club for Liver Cell Biology (ECLCB-8) in October of 2018 in Bonn, Germany.
Identifiants
pubmed: 32188134
pii: ijms21062027
doi: 10.3390/ijms21062027
pmc: PMC7139397
pii:
doi:
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Deutsche Forschungsgemeinschaft
ID : SFB TRR57, CRC1382
Organisme : H2020 European Research Council
ID : 825694
Organisme : H2020 European Research Council
ID : 668031
Organisme : H2020 European Research Council
ID : 731875
Déclaration de conflit d'intérêts
The authors have no conflicts of interest.
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