Connectivity patterns between multiple allergen specific IgE antibodies and their association with severe asthma.

Adolescent Adult Age Factors Aged Allergens / immunology Antibody Specificity / immunology Asthma / diagnosis Biomarkers Body Mass Index Child Child, Preschool Cluster Analysis Europe Female Humans Immunization Immunoglobulin E / immunology Male Middle Aged Prognosis Severity of Illness Index Young Adult

Asthma allergic sensitization cluster network analysis

Journal

The Journal of allergy and clinical immunology

ISSN: 1097-6825

Titre abrégé: J Allergy Clin Immunol

Pays: United States

ID NLM: 1275002

Informations de publication

Date de publication:
10 2020

Historique:

received: 06 05 2019

revised: 23 02 2020

accepted: 27 02 2020

pubmed: 20 3 2020

medline: 16 3 2021

entrez: 20 3 2020

Statut: ppublish

Résumé

Allergic sensitization is associated with severe asthma, but assessment of sensitization is not recommended by most guidelines. We hypothesized that patterns of IgE responses to multiple allergenic proteins differ between sensitized participants with mild/moderate and severe asthma. IgE to 112 allergenic molecules (components, c-sIgE) was measured using multiplex array among 509 adults and 140 school-age and 131 preschool children with asthma/wheeze from the Unbiased BIOmarkers for the PREDiction of respiratory diseases outcomes cohort, of whom 595 had severe disease. We applied clustering methods to identify co-occurrence patterns of components (component clusters) and patterns of sensitization among participants (sensitization clusters). Network analysis techniques explored the connectivity structure of c-sIgE, and differential network analysis looked for differences in c-sIgE interactions between severe and mild/moderate asthma. Four sensitization clusters were identified, but with no difference between disease severity groups. Similarly, component clusters were not associated with asthma severity. None of the c-sIgE were identified as associates of severe asthma. The key difference between school children and adults with mild/moderate compared with those with severe asthma was in the network of connections between c-sIgE. Participants with severe asthma had higher connectivity among components, but these connections were weaker. The mild/moderate network had fewer connections, but the connections were stronger. Connectivity between components with no structural homology tended to co-occur among participants with severe asthma. Results were independent from the different sample sizes of mild/moderate and severe groups. The patterns of interactions between IgE to multiple allergenic proteins are predictors of asthma severity among school children and adults with allergic asthma.

Sections du résumé

BACKGROUND

Allergic sensitization is associated with severe asthma, but assessment of sensitization is not recommended by most guidelines.

OBJECTIVE

We hypothesized that patterns of IgE responses to multiple allergenic proteins differ between sensitized participants with mild/moderate and severe asthma.

METHODS

IgE to 112 allergenic molecules (components, c-sIgE) was measured using multiplex array among 509 adults and 140 school-age and 131 preschool children with asthma/wheeze from the Unbiased BIOmarkers for the PREDiction of respiratory diseases outcomes cohort, of whom 595 had severe disease. We applied clustering methods to identify co-occurrence patterns of components (component clusters) and patterns of sensitization among participants (sensitization clusters). Network analysis techniques explored the connectivity structure of c-sIgE, and differential network analysis looked for differences in c-sIgE interactions between severe and mild/moderate asthma.

RESULTS

Four sensitization clusters were identified, but with no difference between disease severity groups. Similarly, component clusters were not associated with asthma severity. None of the c-sIgE were identified as associates of severe asthma. The key difference between school children and adults with mild/moderate compared with those with severe asthma was in the network of connections between c-sIgE. Participants with severe asthma had higher connectivity among components, but these connections were weaker. The mild/moderate network had fewer connections, but the connections were stronger. Connectivity between components with no structural homology tended to co-occur among participants with severe asthma. Results were independent from the different sample sizes of mild/moderate and severe groups.

CONCLUSIONS

The patterns of interactions between IgE to multiple allergenic proteins are predictors of asthma severity among school children and adults with allergic asthma.

Identifiants

DOI: 10.1016/j.jaci.2020.02.031 PMID: 32188567

pubmed: 32188567

pii: S0091-6749(20)30341-9

doi: 10.1016/j.jaci.2020.02.031

pii:

doi:

Substances chimiques

Allergens 0

Biomarkers 0

Immunoglobulin E 37341-29-0

Types de publication

Journal Article Multicenter Study Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

Pagination

821-830

Subventions

Organisme : Medical Research Council

ID : MR/S025340/1

Pays : United Kingdom