Detection of Bovine Antibodies against a Conserved Capsid Epitope as the Basis of a Novel Universal Serological Test for Foot-and-Mouth Disease.
ELISA
FMDV
conserved capsid
conserved capsid epitope
diagnosis
epitope
foot-and-mouth disease virus
serology
Journal
Journal of clinical microbiology
ISSN: 1098-660X
Titre abrégé: J Clin Microbiol
Pays: United States
ID NLM: 7505564
Informations de publication
Date de publication:
26 05 2020
26 05 2020
Historique:
received:
07
10
2019
accepted:
06
03
2020
pubmed:
20
3
2020
medline:
24
6
2021
entrez:
20
3
2020
Statut:
epublish
Résumé
Diagnostic tests for foot-and-mouth disease (FMD) include the detection of antibodies against either the viral nonstructural proteins or the capsid. The detection of antibodies against the structural proteins (SP) of the capsid can be used to monitor seroconversion in both infected and vaccinated animals. However, SP tests need to be tailored to the individual FMD virus (FMDV) serotype and their sensitivity may be affected by antigenic variability within each serotype and mismatching between test reagents. As a consequence, FMD reference laboratories are required to maintain multiple type-specific SP assays and reagents. A universal SP test would simplify frontline diagnostics and facilitate large-scale serological surveillance and postvaccination monitoring. In this study, a highly conserved region in the N terminus of FMDV capsid protein VP2 (VP2N) was characterized using a panel of intertype-reactive monoclonal antibodies. This revealed a universal epitope in VP2N which could be used as a peptide antigen to detect FMDV-specific antibodies against all types of the virus. A VP2-peptide enzyme-linked immunosorbent assay (VP2-ELISA) was optimized using experimental and reference antisera from immunized, convalescent, and naïve animals (
Identifiants
pubmed: 32188689
pii: JCM.01527-19
doi: 10.1128/JCM.01527-19
pmc: PMC7269384
pii:
doi:
Substances chimiques
Antibodies, Viral
0
Capsid Proteins
0
Epitopes
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/I/00007036
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/L004828/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/I/00007035
Pays : United Kingdom
Informations de copyright
Copyright © 2020 Asfor et al.
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