Mapping p38α mitogen-activated protein kinase signaling by proximity-dependent labeling.
BioID
MAP kinase
biotinylation
interactome
p38
p38α
protein-protein interaction
proximity-dependent labeling
Journal
Protein science : a publication of the Protein Society
ISSN: 1469-896X
Titre abrégé: Protein Sci
Pays: United States
ID NLM: 9211750
Informations de publication
Date de publication:
05 2020
05 2020
Historique:
received:
08
11
2019
revised:
03
03
2020
accepted:
16
03
2020
pubmed:
20
3
2020
medline:
9
2
2021
entrez:
20
3
2020
Statut:
ppublish
Résumé
Mitogen-activated protein (MAP) kinase signaling is central to multiple cellular responses and processes. MAP kinase p38α is the best characterized member of the p38 MAP kinase family. Upstream factors and downstream targets of p38α have been identified in the past by conventional methods such as coimmunoprecipitation. However, a complete picture of its interaction partners and substrates in cells is lacking. Here, we employ a proximity-dependent labeling approach using biotinylation tagging to map the interactome of p38α in cultured 293T cells. Fusing the advanced biotin ligase BioID2 to the N-terminus of p38α, we used mass spectrometry to identify 37 biotin-labeled proteins that putatively interact with p38α. Gene ontology analysis confirms known upstream and downstream factors in the p38 MAP kinase cascade (e.g., MKK3, MAPKAPK2, TAB2, and c-jun). We furthermore identify a cluster of zinc finger (ZnF) domain-containing proteins that is significantly enriched among proximity-labeled interactors and is involved in gene transcription and DNA damage response. Fluorescence imaging and coimmunoprecipitation with overexpressed p38α in cells supports an interaction of p38α with ZnF protein XPA, a key factor in the DNA damage response, that is promoted by UV irradiation. These results define an extensive network of interactions of p38α in cells and new direct molecular targets of MAP kinase p38α in gene regulation and the DNA damage response.
Identifiants
pubmed: 32189389
doi: 10.1002/pro.3854
pmc: PMC7184780
doi:
Substances chimiques
Escherichia coli Proteins
0
Repressor Proteins
0
p38 Mitogen-Activated Protein Kinases
EC 2.7.11.24
Carbon-Nitrogen Ligases
EC 6.3.-
birA protein, E coli
EC 6.3.4.15
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1196-1210Informations de copyright
© 2020 The Protein Society.
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