Microneedle manipulation of the mammalian spindle reveals specialized, short-lived reinforcement near chromosomes.
cell biology
dynamics
kinetochore-fiber
mammalian
mechanics
microneedle
physics of living systems
spindle
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
19 03 2020
19 03 2020
Historique:
received:
21
11
2019
accepted:
18
03
2020
pubmed:
20
3
2020
medline:
5
5
2021
entrez:
20
3
2020
Statut:
epublish
Résumé
The spindle generates force to segregate chromosomes at cell division. In mammalian cells, kinetochore-fibers connect chromosomes to the spindle. The dynamic spindle anchors kinetochore-fibers in space and time to move chromosomes. Yet, how it does so remains poorly understood as we lack tools to directly challenge this anchorage. Here, we adapt microneedle manipulation to exert local forces on the spindle with spatiotemporal control. Pulling on kinetochore-fibers reveals the preservation of local architecture in the spindle-center over seconds. Sister, but not neighbor, kinetochore-fibers remain tightly coupled, restricting chromosome stretching. Further, pulled kinetochore-fibers pivot around poles but not chromosomes, retaining their orientation within 3 μm of chromosomes. This local reinforcement has a 20 s lifetime, and requires the microtubule crosslinker PRC1. Together, these observations indicate short-lived, specialized reinforcement in the spindle center. This could help protect chromosome attachments from transient forces while allowing spindle remodeling, and chromosome movements, over longer timescales.
Identifiants
pubmed: 32191206
doi: 10.7554/eLife.53807
pii: 53807
pmc: PMC7117910
doi:
pii:
Substances chimiques
Tubulin
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIGMS NIH HHS
ID : T32 GM008284
Pays : United States
Organisme : NIGMS NIH HHS
ID : DP2 GM119177
Pays : United States
Organisme : National Science Foundation
ID : 1548297
Organisme : NIGMS NIH HHS
ID : R01 GM134132
Pays : United States
Organisme : National Science Foundation
ID : 1554139
Informations de copyright
© 2020, Suresh et al.
Déclaration de conflit d'intérêts
PS, AL, SD No competing interests declared
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