Extracellular matrix-inducing Sox9 promotes both basal progenitor proliferation and gliogenesis in developing neocortex.
Animals
CRISPR-Cas Systems
Cell Cycle
/ genetics
Cell Differentiation
/ genetics
Cell Proliferation
Extracellular Matrix
/ metabolism
Ferrets
Gene Expression Regulation
Gene Knockdown Techniques
Humans
Mice
Neocortex
/ physiology
Neural Stem Cells
/ cytology
Neurogenesis
/ physiology
Neuroglia
/ cytology
Neurons
/ cytology
SOX9 Transcription Factor
/ genetics
Signal Transduction
developmental biology
ferret
human
mouse
neuroscience
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
19 03 2020
19 03 2020
Historique:
received:
30
06
2019
accepted:
18
03
2020
pubmed:
20
3
2020
medline:
31
3
2021
entrez:
20
3
2020
Statut:
epublish
Résumé
Neocortex expansion is largely based on the proliferative capacity of basal progenitors (BPs), which is increased by extracellular matrix (ECM) components via integrin signaling. Here we show that the transcription factor Sox9 drives expression of ECM components and that laminin 211 increases BP proliferation in embryonic mouse neocortex. We show that Sox9 is expressed in human and ferret BPs and is required for BP proliferation in embryonic ferret neocortex. Conditional Sox9 expression in the mouse BP lineage, where it normally is not expressed, increases BP proliferation, reduces Tbr2 levels and induces Olig2 expression, indicative of premature gliogenesis. Conditional Sox9 expression also results in cell-non-autonomous stimulation of BP proliferation followed by increased upper-layer neuron production. Our findings demonstrate that Sox9 exerts concerted effects on transcription, BP proliferation, neuron production, and neurogenic vs. gliogenic BP cell fate, suggesting that Sox9 may have contributed to promote neocortical expansion.
Identifiants
pubmed: 32191207
doi: 10.7554/eLife.49808
pii: 49808
pmc: PMC7105383
doi:
pii:
Substances chimiques
SOX9 Transcription Factor
0
SOX9 protein, human
0
Banques de données
GEO
['GSE134162', 'GSE38805', 'GSE65000']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Medical Research Council
ID : G0701018
Pays : United Kingdom
Organisme : Medical Research Council
ID : G1100578
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/N004272/1
Pays : United Kingdom
Organisme : Deutsche Forschungsgemeinschaft
ID : SFB 655
Organisme : Deutsche Forschungsgemeinschaft
ID : SFB 655,A2
Organisme : European Research Council
ID : 250197
Pays : International
Organisme : Medical Research Council
ID : MR/R006237/1
Pays : United Kingdom
Organisme : Deutsche Forschungsgemeinschaft
ID : A2
Informations de copyright
© 2020, Güven et al.
Déclaration de conflit d'intérêts
AG, NK, KL, MF, SV, HB, DS, WH No competing interests declared
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