Combined effects of probiotic and chondroprotector during osteoarthritis in rats.
Animals
Arthritis, Experimental
/ drug therapy
Cell Proliferation
/ drug effects
Chondrocytes
/ drug effects
Chondroitin Sulfates
/ pharmacology
Joints
/ drug effects
Male
NF-kappa B
/ metabolism
Osteoarthritis
/ drug therapy
Probiotics
Rats, Wistar
Toll-Like Receptor 2
/ metabolism
Toll-Like Receptor 4
/ metabolism
Tumor Necrosis Factor-alpha
/ metabolism
Journal
Panminerva medica
ISSN: 1827-1898
Titre abrégé: Panminerva Med
Pays: Italy
ID NLM: 0421110
Informations de publication
Date de publication:
Jun 2020
Jun 2020
Historique:
pubmed:
21
3
2020
medline:
7
4
2021
entrez:
21
3
2020
Statut:
ppublish
Résumé
Osteoarthritis (OA) is a joint affection, defined by articular cartilage demolition, risks of which rise with age. The aim of this study was to compare the efficacy of chondroitin sulfate (CS) course and multistrain live probiotic (LP) administered alone or in combination on the expression of TLR-2, TLR-4, TNF-α and NF-κB in articular cartilage, subchondral bone and synovial membrane during OA in rats. OA was induced in male rats by injecting monoiodoacetate (MIA) in right hind knee. Therapeutic groups received 3 mg/kg of chondroprotector (ChP) CS for 28 days and/or 140 mg/kg of LP diet for 14 days. The expression of TLR-2, TLR-4, TNF-α and NF-κB in articular cartilage, subchondral bone and synovial membrane were determined with immunohistochemical staining kits (Thermo Fisher Scientific). It was established that MIA injection is associated with long-term structural changes in joint tissues that corresponded to OA-like features and associated with activation of pathogen-recognizing molecules and proinflammatory signaling pathways expression. Separate therapy with ChP and probiotics slightly decreased OA score limiting cell death and subchondral bone resorption. However, these changes were not associated with a significant decrease in TLR-2, TLR-4, NF-kB and TNF-α expression. On the other hand, the combination of ChP and LP treatment significantly decreased OA score. This correlated with a decrease in TLR-2, TLR-4, NF-kB and TNF-α expression in chondrocytes and synovial cells. The outcomes of our research prove that ChPs amplify the positive action of LPs in OA attenuation.
Sections du résumé
BACKGROUND
BACKGROUND
Osteoarthritis (OA) is a joint affection, defined by articular cartilage demolition, risks of which rise with age. The aim of this study was to compare the efficacy of chondroitin sulfate (CS) course and multistrain live probiotic (LP) administered alone or in combination on the expression of TLR-2, TLR-4, TNF-α and NF-κB in articular cartilage, subchondral bone and synovial membrane during OA in rats.
METHODS
METHODS
OA was induced in male rats by injecting monoiodoacetate (MIA) in right hind knee. Therapeutic groups received 3 mg/kg of chondroprotector (ChP) CS for 28 days and/or 140 mg/kg of LP diet for 14 days. The expression of TLR-2, TLR-4, TNF-α and NF-κB in articular cartilage, subchondral bone and synovial membrane were determined with immunohistochemical staining kits (Thermo Fisher Scientific).
RESULTS
RESULTS
It was established that MIA injection is associated with long-term structural changes in joint tissues that corresponded to OA-like features and associated with activation of pathogen-recognizing molecules and proinflammatory signaling pathways expression. Separate therapy with ChP and probiotics slightly decreased OA score limiting cell death and subchondral bone resorption. However, these changes were not associated with a significant decrease in TLR-2, TLR-4, NF-kB and TNF-α expression. On the other hand, the combination of ChP and LP treatment significantly decreased OA score. This correlated with a decrease in TLR-2, TLR-4, NF-kB and TNF-α expression in chondrocytes and synovial cells.
CONCLUSIONS
CONCLUSIONS
The outcomes of our research prove that ChPs amplify the positive action of LPs in OA attenuation.
Identifiants
pubmed: 32192320
pii: S0031-0808.20.03841-0
doi: 10.23736/S0031-0808.20.03841-0
doi:
Substances chimiques
NF-kappa B
0
Tlr2 protein, rat
0
Tlr4 protein, rat
0
Toll-Like Receptor 2
0
Toll-Like Receptor 4
0
Tumor Necrosis Factor-alpha
0
Chondroitin Sulfates
9007-28-7
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM