Class II phosphatidylinositol 3-kinase α and β isoforms are required for vascular smooth muscle Rho activation, contraction and blood pressure regulation in mice.
Blood pressure
Class II phosphatidylinositol 3-kinase
Contraction
Rho
Vascular smooth muscle
Journal
The journal of physiological sciences : JPS
ISSN: 1880-6562
Titre abrégé: J Physiol Sci
Pays: Japan
ID NLM: 101262417
Informations de publication
Date de publication:
19 Mar 2020
19 Mar 2020
Historique:
received:
22
01
2020
accepted:
10
03
2020
entrez:
21
3
2020
pubmed:
21
3
2020
medline:
20
2
2021
Statut:
epublish
Résumé
Class II phosphatidylinositol 3-kinases (PI3K), PI3K-C2α and PI3K-C2β, are involved in cellular processes including endocytosis, cilia formation and autophagy. However, the role of PI3K-C2α and PI3K-C2β at the organismal level is not well understood. We found that double knockout (KO) mice with both smooth muscle-specific KO of PI3K-C2α and global PI3K-C2β KO, but not single KO mice of either PI3K-C2α or PI3K-C2β, exhibited reductions in arterial blood pressure and substantial attenuation of contractile responses of isolated aortic rings. In wild-type vascular smooth muscle cells, double knockdown of PI3K-C2α and PI3K-C2β but not single knockdown of either PI3K markedly inhibited contraction with reduced phosphorylation of 20-kDa myosin light chain and MYPT1 and Rho activation, but without inhibition of the intracellular Ca
Identifiants
pubmed: 32192434
doi: 10.1186/s12576-020-00745-2
pii: 10.1186/s12576-020-00745-2
pmc: PMC7082390
doi:
Substances chimiques
Isoenzymes
0
Class II Phosphatidylinositol 3-Kinases
EC 2.7.1.137
rho GTP-Binding Proteins
EC 3.6.5.2
Calcium
SY7Q814VUP
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
18Subventions
Organisme : Ministry of Education, Culture, Sports, Science and Technology
ID : 25116711
Organisme : Japan Society for the Promotion of Science
ID : 17K08532
Organisme : Japan Society for the Promotion of Science
ID : 16K18988
Organisme : Japan Society for the Promotion of Science
ID : 16K15409
Organisme : Japan Society for the Promotion of Science
ID : 17K08542
Organisme : Japan Society for the Promotion of Science
ID : 15H04673
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