Cell-Type-Specific Adhesiveness and Proliferation Propensity on Laminin Isoforms Enable Purification of iPSC-Derived Corneal Epithelium.
Cell Adhesion
/ drug effects
Cell Differentiation
/ drug effects
Cell Proliferation
/ drug effects
Cell Separation
/ methods
Cells, Cultured
Epithelial Cells
/ cytology
Epithelium, Corneal
/ cytology
Humans
Induced Pluripotent Stem Cells
/ cytology
Integrins
/ metabolism
Laminin
/ pharmacology
Protein Isoforms
/ pharmacology
cell competition
cell purification
corneal epithelial cells (CECs)
human induced pluripotent stem cells (hiPSCs)
laminin isoforms
substrate-specific adhesion
Journal
Stem cell reports
ISSN: 2213-6711
Titre abrégé: Stem Cell Reports
Pays: United States
ID NLM: 101611300
Informations de publication
Date de publication:
14 04 2020
14 04 2020
Historique:
received:
07
05
2019
revised:
20
02
2020
accepted:
24
02
2020
pubmed:
21
3
2020
medline:
7
2
2021
entrez:
21
3
2020
Statut:
ppublish
Résumé
A treatment for intractable diseases is expected to be the replacement of damaged tissues with products from human induced pluripotent stem cells (hiPSCs). Target cell purification is a critical step for realizing hiPSC-based therapy. Here, we found that hiPSC-derived ocular cell types exhibited unique adhesion specificities and growth characteristics on distinct E8 fragments of laminin isoforms (LNE8s): hiPSC-derived corneal epithelial cells (iCECs) and other non-CECs rapidly adhered preferentially to LN332/411/511E8 and LN211E8, respectively, through differential expression of laminin-binding integrins. Furthermore, LN332E8 promoted epithelial cell proliferation but not that of the other eye-related cells, leading to non-CEC elimination by cell competition. Combining these features with magnetic sorting, highly pure iCEC sheets were fabricated. Thus, we established a simple method for isolating iCECs from various hiPSC-derived cells without using fluorescence-activated cell sorting. This study will facilitate efficient manufacture of iCEC sheets for corneal disease treatment and provide insights into target cell-specific scaffold selection.
Identifiants
pubmed: 32197114
pii: S2213-6711(20)30064-3
doi: 10.1016/j.stemcr.2020.02.008
pmc: PMC7160305
pii:
doi:
Substances chimiques
Integrins
0
Laminin
0
Protein Isoforms
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
663-676Informations de copyright
Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.
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